PEG处理葡萄试管苗生理反应及叶片组织形态研究

应用不同浓度的聚乙二醇(PEG10000)处理红地球葡萄生根试管苗,在造成一定程度水分亏缺的情况下,观察葡萄试管苗的生理反应和植株生长、叶片形态解剖结构的变化。结果表明:在PEG处理下,葡萄生长发育迟滞,植株节间变短,株高变矮,但发根数量增加,根变长;新生叶片与对照比,叶片小而皱缩,气孔指数降低,上皮细胞排列更紧凑;同时葡萄试管苗脯氨酸的积累和内源ABA迅速增加。说明在一定强度的水分胁迫下,葡萄试管苗叶片的发育向增强其耐旱能力的方向进行,其渗透调节物质的积累是葡萄试管苗对长期水分胁迫的生理反应。

Study on physiological metabolic indexes and leaf organic morphology of grape plantlets in vitro under PEG-indued water stress

The physiological indexes, plant growth and leaf organic morphology of grape plantlets in vitro under water stress induced by polyethylene glycal (PEG) with different concentrations have been observed and analysed. The results indicate that, under PEG treatment, the growth of plantlets is inhibited, the internode length and plant height are shortened, while the amount and length of roots are increased; the newly-developed leaves become smaller and crimple, their stomatal index is reduced, and the epidermal cells are collocated more closely; the proline accumulation and ABA content in leaves of the plantlets are raised rapidly. It can be concluded that a moderate drought stress may enhence the acclimation of grape leaves to water deficit by changing leaf ontogeny process, and that the accumulation of osmotic active matter may be certain physiological responses of grape to long-term water stress.