| 桑椹与叶片蛋白质双向电泳样品制备方法的比较试验 |
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| 引用本文： | 王海玲,池旭娟,阚雪芹,方向明,刘艳艳,谈建中.桑椹与叶片蛋白质双向电泳样品制备方法的比较试验[J].蚕业科学,2009,35(4). |
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| 作者姓名： | 王海玲 池旭娟 阚雪芹 方向明 刘艳艳 谈建中 |
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| 作者单位： | 苏州大学建筑与城市环境学院,江苏苏州,215123;苏州大学建筑与城市环境学院,江苏苏州,215123;苏州大学蚕桑研究所,江苏苏州,215123 |
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| 基金项目： | 苏州市农业科技计划项目,公益性行业(农业)科研专项 |
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| 摘 要： | 为建立适合于桑树蛋白质组研究的双向电泳技术体系,以桑品种"大10"的桑椹与叶片为材料,探讨了桑树蛋白质不同提取方法对凝胶电泳效果的影响。结果表明,改良酚提取法与常规的SDS提取法、尿素/硫脲提取法相比,电泳图谱清晰,分离效果良好,能检测到更多的蛋白质组分,适用于桑椹与叶片蛋白质IPG-IEF/SDS-PAGE双向电泳及SDS-PAGE电泳的样品制备。
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| 关 键 词： | 桑椹 桑叶 双向电泳 蛋白质样品 |
Comparative Tests on Preparation Method of Protein Samples From Mulberry Fruit and Leaf for Two-dimensional Electrophoresis |
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| WANG Hai-Ling,CHI Xu-Juan,KAN Xue-Qin,FANG Xiang-Ming,LIU Yan-Yan,TAN Jian-Zhong.Comparative Tests on Preparation Method of Protein Samples From Mulberry Fruit and Leaf for Two-dimensional Electrophoresis[J].Acta Sericologica Sinica,2009,35(4). |
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| Authors: | WANG Hai-Ling CHI Xu-Juan KAN Xue-Qin FANG Xiang-Ming LIU Yan-Yan TAN Jian-Zhong |
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| Institution: | WANG Hai-Ling1 CHI Xu-Juan1 KAN Xue-Qin1 FANG Xiang-Ming1LIU Yan-Yan1 TAN Jian-Zhong1,2(1School of Architecture , Urban Environment,Soochow University,Suzhou Jiangsu 215123,China,2Institute of Sericulture,China) |
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| Abstract: | In order to develop two-dimensional electrophoresis technology suitable for mulberry proteome analysis,the effects of various protein extraction methods on gel electrophoresis were investigated by using fruit and leaves of a mulberry variety named Da 10.The results showed that the modified phenol extraction method could yield clearer electrophoretogram and better separation effect over the conventional SDS extraction method and urea/thiourea extraction method.With the modified phenol method,more protein com... |
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| Keywords: | Mulberry fruit Mulberry leaf Two-dimensional electrophoresis (2-DE) Protein sample preparation |
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