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| 应用电镜技术快速检测培养细胞中的支原体污染 | |
| 引用本文： | 李六金,姜焕宏,李成,刘建荣,马伟辉,李宏. 应用电镜技术快速检测培养细胞中的支原体污染[J]. 中国兽药杂志, 2002, 36(6): 31-33 |
| 作者姓名： | 李六金 姜焕宏 李成 刘建荣 马伟辉 李宏 |
| 作者单位： | 1. 第四军医大学实验动物研究中心,陕西西安,710032 2. 西安市现代农业开发总公司,陕西西安,710021 3. 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001 |
| 摘 要： | 应用电镜技术可快速准确地检测培养细胞中的支原体污染。通过高速离心浓缩样品,负染色后电镜观察,可在30min内做出判定,方法快速、简便、准确。 |
| 关 键 词： | 电镜技术 支原体 培养细胞 |
| 文章编号： | 1002-1280(2002)06-0030-03 |
| 修稿时间： | 2001-11-30 |
Rapid Detection for the Mycoplasma Contamination of Cultural Cells with Electron Microscopy |
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| LI Liu-jin,JIANG Huan-hong,LI Cheng ,LIU Jian-rong,MA Wei-hui,LI Hong. Rapid Detection for the Mycoplasma Contamination of Cultural Cells with Electron Microscopy[J]. Chinese Journal of Veterinary Drug, 2002, 36(6): 31-33 | |
| Authors: | LI Liu-jin JIANG Huan-hong LI Cheng LIU Jian-rong MA Wei-hui LI Hong |
| Affiliation: | LI Liu-jin1,JIANG Huan-hong2,LI Cheng 3,LIU Jian-rong1,MA Wei-hui1,LI Hong1 |
| Abstract: | The mycoplama contamination of cultural cells could be rapidly and accurately detected with electronmicroscopy. The concentrates were obtained by the superspeed centrifuge, which were observed with electronmicroscopy after negetive stain, and judged in 30 minutes. |
| Keywords: | electron microscopy mycoplama cultural cells |
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