珍稀濒危植物沙冬青的组织培养

试验以珍稀濒危植物沙冬青无菌苗的子叶、茎段为外植体材料,研究不同激素配比的培养基对不同外植体愈伤组织培养、丛生芽增殖及生根培养的影响,并筛选适宜各阶段培养的最佳培养基配方。结果表明,子叶是诱导沙冬青愈伤组织的最好外植体,在6-BA 0.5 mg/L、2,4-D 0.5 mg/L时,其愈伤组织诱导率达到最大值81.8%;在6-BA 2.0 mg/L、NAA 0.5 mg/L时丛生芽增殖率达到最大值90.2%,且丛生芽数量多、茎干健壮、生长旺盛,说明较高浓度的细胞分裂6-BA对丛生芽增殖具有明显的促进作用;在以1/2MS为基本培养基,加入NAA 1.0 mg/L时,生根率达到最大值70.4%,且生根时间最早,主根明显、粗壮且根毛数量多;适当调节培养基中无机盐的浓度提高生根率是将来沙生植物组织培养中研究的重点和难点。

Tissue Culture of Endangered Psammophyte Ammopiptanthus mongolicus

The paper aims to test effects of medium with different hormone combinations on explants callus culture, buds proliferation and rooting culture of rare and endangered plants Ammopiptanthus with aseptic cotyledons and stem segments as explants materials, and screen the optimum medium formula for each culture stage. The results showed that cotyledon was the best explants to induct callus of Ammopiptanthus. The callus induction rate reached its maximum 81.8% at 6-BA 0.5 mg/L, 2,4-D 0.5 mg/L. The clustered buds proliferation rate reached the maximum of 90.2% at 6-BA 2.0 mg/L, NAA 0.5 mg/L. The phenomenon of multiple clustered buds, robust stem and vigorous growth was observed, indicating that higher concentrations of cell division 6-BA could significantly promote cluster bud proliferation. The rooting rate reached the maximum of 70.4% in 1/2MS basic medium added with NAA 1.0 mg/L, the rooting time was the earliest, with obvious main root, which was stout and hairy. The key point in psammophytes tissue culture research will be adjusting inorganic salt concentration in the medium properly to improve the rooting rate.