| 多重PCR方法检测食品中霍乱弧菌、副溶血性弧菌和单核细胞增生李斯特氏菌 |
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| 引用本文： | 江迎鸿,谭贵良,陈亚波,李向丽. 多重PCR方法检测食品中霍乱弧菌、副溶血性弧菌和单核细胞增生李斯特氏菌[J]. 广东农业科学, 2011, 38(10): 135-137,150 |
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| 作者姓名： | 江迎鸿 谭贵良 陈亚波 李向丽 |
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| 作者单位： | 1. 中山市质量计量监督检测所,广东中山,528403
2. 中山火炬职业技术学院生物医药系,广东中山,528436 |
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| 基金项目： | 中山市科技计划项目(20083A279) |
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| 摘 要： | 通过扩增霍乱弧菌(VC)、副溶血性弧菌(VP)和单核细胞增生李斯特氏菌(LM)的特异性核酸片段,建立了VC、VP和LM的多重PCR检测方法,相应的扩增片段分别为588、450、234 bp。该方法特异性强、灵敏度高、简便、快速,可实现对上述致病菌的同时检测,在接种食品中检测灵敏度达到103 CFU/mL。
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| 关 键 词： | 多重PCR 霍乱弧菌 副溶血性弧菌 单核细胞增生李斯特氏菌 食品 |
Simultaneous detection of three food-borne bacterial pathogens by mutiplex PCR |
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| JIANG Ying-hong,TAN Gui-liang,CHEN Ya-bo,LI Xiang-li. Simultaneous detection of three food-borne bacterial pathogens by mutiplex PCR[J]. Guangdong Agricultural Sciences, 2011, 38(10): 135-137,150 |
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| Authors: | JIANG Ying-hong TAN Gui-liang CHEN Ya-bo LI Xiang-li |
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| Affiliation: | JIANG Ying-hong1,TAN Gui-liang1,CHEN Ya-bo1,LI Xiang-li2(1.Zhongshan Supervision Testing Institute of Quality and Metrology,Zhongshan 528403,China,2.Department of Biomedicine,Zhongshan Torch Technology College,Zhongshan 528436,China) |
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| Abstract: | A multiplex PCR method for detecting three food-borne pathogens(Vibrio cholerae,VC;Vibrio parahaemolyticus,VP;Listeria monocytogenes,LM) was developed,based on their specific nucleic acid fragments.The amplification productions of VC,VP and LM were 588 bp,450 bp and 234 bp,respectively.The mutiplex PCR assay was a highly specific,sensitive and rapid method,which could simultaneously determine VC,VP and LM in spiked food.The detection limit was 103 CFU/mL. |
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| Keywords: | mutiplex PCR Vibrio cholerae Vibrio parahaemolyticus Listeria monocytogenes food |
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