Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells |
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| Authors: | Giuliano Pisoni Patrizia D’Amelio Francesca Sassi Giovanni Manarolla Licia Scaccabarozzi Clara Locatelli Nausicaa Mazzocchi Mirko Baruscotti Dario DiFrancesco Paolo Moroni |
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| Institution: | aUniversità degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, via Celoria 10, 20133 Milano, Italy;bUniversità degli Studi di Torino, Dipartimento di Discipline Mediche e Chirurgiche, Sezione di Geriatria, Torino, Italy;cUniversità degli Studi di Milano, Dipartimento di Scienze Biomolecolari e Biotecnologie, Laboratorio di Fisiologia Molecolare e Neurobiologia, via Celoria 26, 20133 Milano, Italy |
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| Abstract: | Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7–14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype. |
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| Keywords: | Caprine Multinucleated giant cells Peripheral blood mononuclear cells Osteoclast |
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