| 用RAPD标记分析部分柞蚕二化性品种资源的遗传多样性 |
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| 引用本文： | 王凤成,刘丹梅,仝振祥,冀万杰,张博.用RAPD标记分析部分柞蚕二化性品种资源的遗传多样性[J].蚕业科学,2009,35(1). |
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| 作者姓名： | 王凤成 刘丹梅 仝振祥 冀万杰 张博 |
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| 作者单位： | 辽宁省蚕业科学研究所,辽宁,凤城,118100;辽东学院,辽宁丹东,118000 |
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| 摘 要： | 采用RAPD标记技术,对36份柞蚕(Antheraea pernyi)二化性品种资源的遗传多样性进行分析。PCR扩增及琼脂糖凝胶电泳结果表明:利用筛选出的19个随机引物在供试材料中共检测到123个信息位点,其中多态性位点114个,多态性比率92.68%;每一个引物可检测出3～9个位点,其多态性比率不尽相同,平均扩增出6.47条带;各品种具有不同数量的特异性带型。供试品种资源的Nei氏遗传多样性指数(h)为0.324 3±0.151 5,Shannon信息指数(I)为0.487 1±0.197 5,表明长期同地保存的二化性柞蚕品种资源同样具有丰富的遗传多样性;供试品种的Nei氏遗传一致性范围在0.495 9～0.837 4之间,达0.6以上的占96.59%,表明多数品种资源的相似程度较高。36份品种资源分别以辽宁省蚕业科学研究所育成种为主和以国内外引进种为主聚为两大类群。
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| 关 键 词： | 柞蚕 品种资源 遗传多样性 随机扩增多态DNA |
RAPD Analysis on Genetic Diversity of Bivoltine Tussah Silkworm Varieties |
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| WANG Feng-Cheng,LIU Dan-Mei,TONG Zhen-Xiang,JI Wan-Jie,ZHANG Bo.RAPD Analysis on Genetic Diversity of Bivoltine Tussah Silkworm Varieties[J].Acta Sericologica Sinica,2009,35(1). |
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| Authors: | WANG Feng-Cheng LIU Dan-Mei TONG Zhen-Xiang JI Wan-Jie ZHANG Bo |
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| Institution: | WANG Feng-Cheng1 LIU Dan-Mei2 TONG Zhen-Xiang1 JI Wan-Jie1 ZHANG Bo1(1Sericulture Research Institute of Liaoning Province,Fengcheng Liaoning 118100,China,2Eastern Liaoning University,D,ong Liaoning 118000,China) |
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| Abstract: | The genetic diversity of 36 bivoltine tussah silkworm varieties were analyzed by means of RAPD.The result of PCR amplification and agarose gel electrophoresis showed that 123 loci were detected in DNA samples of 36 tussah silkworm varieties by 19 randomly selected primers,among which 114 loci are polymorphic,accounting for 92.68% of all the loci detected.For each primer,3 to 9 loci can be detected with different polymorphic ratio.On average,6.47 bands could be amplified by each primer.Of the 36 tussah silkw... |
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| Keywords: | Antheraea pernyi Germplasm resources Genetic diversity Random amplified polymorphic DNA |
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