| 响应面法优化茯苓菌丝体多糖的提取工艺研究 |
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| 引用本文： | 陶跃中,姜辉,舒志愚,杨生玉.响应面法优化茯苓菌丝体多糖的提取工艺研究[J].安徽农业科学,2010,38(17):9095-9097,9364. |
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| 作者姓名： | 陶跃中 姜辉 舒志愚 杨生玉 |
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| 作者单位： | 1. 河南大学生物工程研究所,河南开封,475004
2. 江苏延申生物科技股份有限公司,江苏常州,213100 |
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| 基金项目： | 河南省科技攻关重点项目 |
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| 摘 要： | 目的]优化茯苓深层发酵菌丝体的多糖提取工艺。方法]在单因素试验基础上,根据Box-Benhnken的中心组合试验设计原理,选取提取时间、提取温度和水料比3因素3水平的响应面法优化茯苓多糖的提取工艺。结果]提取时间、提取温度以及水料比与茯苓多糖得率存在显著相关性（P〈0.05）;茯苓多糖水浸提最佳工艺条件为：提取时间4.3 h,提取温度73.8℃,水料比29.8∶1;多糖得率理论值达到2.45%,实际得率可达2.57%。结论]采用响应面法优化工艺得到的提取条件可信,具有可行性和应用价值。
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| 关 键 词： | 茯苓多糖 提取 响应面法 优化 |
Optimization of Processing Parameters for Extraction of Soluble Poria cocos Polysaccharides by Response Surface Methodology |
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| TAO Yue-zhong et al.Optimization of Processing Parameters for Extraction of Soluble Poria cocos Polysaccharides by Response Surface Methodology[J].Journal of Anhui Agricultural Sciences,2010,38(17):9095-9097,9364. |
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| Authors: | TAO Yue-zhong |
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| Institution: | TAO Yue-zhong et al(College of Life Science,Henan University,Kaifeng,Henan 475004) |
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| Abstract: | Objective]To optimize the extraction conditions for the yield of Poria cocos polysaccharides.Method] Based on the results of single factor tests,a three-level Box-Behnken factorial design was employed combining with response surface methodology to optimize the extraction conditions for the yield of Poria cocos polysaccharides.Result] A mathematical model was then developed to show the significant effects of each factor and their interactions on the yield of Poria cocos polysaccharides.A maximal yield of ... |
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| Keywords: | Poria cocos polysaccharides Extraction Response surface methodology Optimization |
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