大果黑果枸杞组培快繁技术体系研究

[目的]该研究旨在建立大果黑果枸杞组培快繁技术体系。[方法]以大果黑果枸杞嫩茎作为外植体，以 MS为基本培养基，研究了不同因素对大果黑果枸杞组培苗初代、继代以及生根培养的影响。[结果]适合大果黑果枸杞初代培养的培养基配方为 MS+ZT 0.2 mg/L+IBA 0.01 mg/L，在此培养基上，腋芽的生长状况良好，萌芽率高达88.73%，且玻璃化的情况很少；同样， ZT对组培苗增殖培养的效果要优于6-BA，适合组培苗增殖培养的培养基配方为MS+ZT 0.15 mg/L+ IBA 0.01 mg/L，在此培养基上，组培苗的生长速度快，其增殖倍数可达5.83倍，组培苗基本没有玻璃化现象，且苗生长健壮；适合组培苗生根的培养基为 MS+IBA1.0 mg/L,生根率可达100%。[结论]适合大果黑果枸杞组培苗炼苗移栽的基质配比为腐殖土∶珍珠岩=1∶1，在此基质中培养的苗木均长势良好，成活率高达92.37%。[结论]该研究为大果黑果枸杞的规模化生产及推广提供理论依据。

Study on Tissue Culture and Rapid Propagation of Lycium ruthenicum Murr.

Objective] This study aimed to establish a tissue culture and rapid propa-gation system for Lycium ruthenicum Murr. [Method] Using tender stems of L. ruthenicum as explants, MS as basic culture medium, the effects of different factors on primary culture, subculture and rooting of L. ruthenicum plantlets were investigat-ed. [Result] The most appropriate medium for primary culture of L. ruthenicum was MS + ZT 0.2 mg/L + IBA 0.01 mg/L, in which axil ary buds grew wel and were rarely vitrified with the germination rate of 88.73%. In addition, ZT exerted significant-ly better effects on subculture and proliferation of L. ruthenicum plantlets than 6-BA. The most appropriate medium for subculture and proliferation of L. ruthenicum plantlets was MS + ZT 0.15 mg/L + IBA 0.01 mg/L, in which L. ruthenicum plantlets grew rapidly and robustly without vitrification, and the proliferation multiple reached 5.83 times. The most appropriate medium for rooting of L. ruthenicum plantlets was MS + IBA 1.0 mg/L, in which the rooting rate reached 100%. The most appropriate substrate for transplanting and hardening of L. ruthenicum plantlets was humus soil: perlite= 1:1, in which L. ruthenicum plantlets grew wel with the survival rate of 92.37%. [Conclusion] This study provided theoretical basis for large-scale production and popularization of L. ruthenicum.