| 大豆制品转基因成分检测和定量标识分析 |
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| 引用本文： | 董流昕,谢响明,毛建军,陈红运,陈洪俊,朱水芳.大豆制品转基因成分检测和定量标识分析[J].植物检疫,2005,19(3):132-134. |
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| 作者姓名： | 董流昕 谢响明 毛建军 陈红运 陈洪俊 朱水芳 |
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| 作者单位： | 1. 北京林业大学生物科学与技术学院,100083
2. 中国检验检疫科学研究院动植物检疫研究所 |
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| 摘 要： | 分别以大豆凝集素(Lectin)和5-莽草酸-3磷酸合成酶(CP4-EPSPS)为内源和目标基因,对28种大豆制品进行实时荧光PCR检测.检测结果表明,4种大豆制品的CP4-EPSPS扩增结果为阳性,含有转基因大豆成分.转基因成分的定量检测在贸易和可操作性方面要优于定性检测.
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| 关 键 词： | 实时荧光PCR 转基因 大豆制品 |
Detection of soybean produts of genetically modified components and quantitatively labeling analysis |
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| Dong liuxin,Xie Xiangming,Mao jianjun,Chen hongyuu,Cheng hongjun,Zhu Shuifang.Detection of soybean produts of genetically modified components and quantitatively labeling analysis[J].Plant Quarantine,2005,19(3):132-134. |
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| Authors: | Dong liuxin Xie Xiangming Mao jianjun Chen hongyuu Cheng hongjun Zhu Shuifang |
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| Abstract: | Twenty-eight kinds of soybean products were detected by real-time fluorescent PCR using soybean lectin which was soybean endogenesis reference gene and target gene 5-enolpyruvylshikimate-3-phosphate synthase g ene (CP4-EPSPS) which was induced herbicide tolerance in the modified soybean . The result shows that four of them were positive,which contain genetically modi fied soybean components.Quantitative detection of genetically modified organism components is superior to quality. |
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| Keywords: | Real-time fluorescent PCR Genetically modified org anism Soybean products |
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