Using BCG,MPT-51 and Ag85 as antigens in an indirect ELISA for the diagnosis of bovine tuberculosis |
| |
Authors: | Ediane Batista da Silva Bruna Daniella de Souza Silva Jerônimo Rafael Ruiz Leon André Kipnis Isabel KF de Miranda Santos Ana Paula Junqueira-Kipnis |
| |
Institution: | 1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;2. Veterinary Council of Khorasan Razavi, Mashad, Iran |
| |
Abstract: | This study evaluated the Mycobacterium tuberculosis protein antigen MPT-51, the trimeric antigen 85 (Ag85) complex, and Bacillus Calmette-Guérin (BCG) in an indirect ELISA to diagnose bovine tuberculosis (TB) from serum samples. Serum was collected from 208 intra-dermal tuberculin test (ITT)-positive and 54 ITT-negative animals from a region where bovine TB is endemic. Using the Ag85 and BCG antigens, the indirect ELISA was able to discriminate ITT-positive from ITT-negative animals. This level of discrimination was not achieved when using the MPT-51 antigen. The highest sensitivity (Se) and specificity (Sp) of the test was found when BCG was used (Se, 82%; Sp, 91%). Further work in different epidemiological settings and with larger numbers of animals will be required to validate these findings. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|