铁皮石斛组培苗移栽基质的筛选

组培快繁是目前铁皮石斛种苗生产的主要途径,组培苗移栽成活率和后期生长势是决定组培快繁生产种苗是否取得最后成功的关键环节。移栽基质是影响组培苗移栽成功的重要因素,尤其对于铁皮石斛这种对栽培基质的水分、通气、营养等状况要求比较严格的附生兰而言,其作用尤为突出。评价了不同基质配比对铁皮石斛组培苗移栽成活率和后期生长势的影响,旨在筛选、优化移栽基质,以提高铁皮石斛种苗的成苗率与产量,降低生产成本,更好地满足铁皮石斛种苗规模化生产的需求。研究结果表明,移栽365d后铁皮石斛在基质配比泥炭∶树皮∶刨花=2∶4∶4上的成活率为95.6%,平均萌芽数达到8.2个、株高52.16mm、茎粗3.47mm;显著优于其在纯细锯末、泥炭∶树皮∶刨花=3∶3∶4和泥炭∶树皮∶刨花=2∶3∶5上的表现,因此,筛选出泥炭∶树皮∶刨花=2∶4∶4的基质配比作为铁皮石斛组培苗移栽的最佳基质。

Screening of Matrix for in vitro Plantlet Transplantation of Dendrobium officinde

In vitro micropropagation is a major way for massive production of plantlets of Dendrobium officinale.The ultimate success of this plantlet production undoubtedly depended on the survival and vigorous growth during and after transplantation.Matrix plays a significant role during the above process of Dendrobium officinale,which is extremingly strict with water,aeration and nutrition.Aimed at matrix optimizing,the effects of matrix type and combination on survival rate and subsequent growth vigor of transplanted plantlets of Dendrobium officinale were assessed in present study.The results indicated that the combination of peat,bark,and paring with proportion of 2∶2∶4 produced higher survival rate(95.6%)and more vigorous growth(got 8.2buds,52.16mm height,3.47mm diameter in average)obviously than other treatment after transplantation of 365 d.Thus the treatment of peat,bark and paring(2∶2∶4)was the optimized matrix for in vitro plantlet transplantation of Dendrobium officinale.