| 梭鲈的淋巴细胞培养及其核型研究 |
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| 引用本文： | 黄金善,范兆廷.梭鲈的淋巴细胞培养及其核型研究[J].东北农业大学学报,2012,43(3):104-109. |
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| 作者姓名： | 黄金善 范兆廷 |
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| 作者单位： | 东北农业大学动物科学技术学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省科技攻关项目(GA06B203) |
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| 摘 要： | 采用RPMI l640全培养基对梭鲈(Sander luciopercaLinnaeus)淋巴细胞进行体外培养,经空气干燥制备染色体玻片标本并对其核型进行分析。结果表明,梭鲈染色体数目为48条,包括2条中部着丝点染色体、10条亚中部着丝点染色体、12条亚端部着丝粒染色体和24条端部着丝粒染色体,未发现异型性染色体对和随体。梭鲈核型公式为2n=48,2m+10sm+12st+24t,其染色体总臂数(NF)为60。
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| 关 键 词： | 梭鲈 淋巴细胞 核型 |
Study of peripheral blood cell cultivetion and karyotype of Sander lucioperca Linnaeus |
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| HUANG Jinshan
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FAN Zhaoting.Study of peripheral blood cell cultivetion and karyotype of Sander lucioperca Linnaeus[J].Journal of Northeast Agricultural University,2012,43(3):104-109. |
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| Authors: | HUANG Jinshan FAN Zhaoting |
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| Institution: | (College of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The blood cell of Sander lucioperca was cultured(Linnaeus)by the whole culture medium of RPMI 1640 in vitro.By evaluating the content of colchicines and permeating time,establish a set of mature experimental method to cultivate the blood lymphocytic cell and prepare chromosome of pike-perch.Metaphase chromosome specimens of Sander lucioperca Linnaeus were prepared from short-term culture of peripheral blood lymphocytes with air-drying technique.Experimental results indicated that the diploid chromosome number was 2n=48 and the karyotype was compose of 2m,10sm,10st,12t,NF=60. |
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| Keywords: | Sander lucioperca Linnaeus peripheral blood karyotype |
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