| 君子兰八氢番茄红素合成酶基因的克隆与序列分析 |
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| 引用本文： | 劳杉杉,苑志明,秦智伟,周秀艳.君子兰八氢番茄红素合成酶基因的克隆与序列分析[J].东北农业大学学报,2012,43(4):113-117. |
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| 作者姓名： | 劳杉杉 苑志明 秦智伟 周秀艳 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
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| 基金项目： | 国家农业科技成果转化资金项目，黑龙江省高校科技创新团队项目，黑龙江省蔬菜生物学重点实验室项目 |
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| 摘 要： | 根据GenBank上登陆的黄水仙八氢番茄红素合成酶基因(登录号:X78814.1)设计特异引物,通过PCR扩增从君子兰cDNA中克隆出一个1.4 kb的片段。序列分析表明,这个序列全长1 395 bp,包含一个1 272 bp的开放阅读框,并且编码423个氨基酸。与水仙(DQ984674)、文心兰(FJ859988)和茶花(EF545005)相比,此cDNA序列显示出98%~76%的同源性,并且与水仙、猕猴桃、番茄和柿相比,其氨基酸序列显示出达到了99%~80%的同源性。由此可知,研究所克隆的序列为君子兰的PSY全长基因,该PSY基因已登陆GenBank(登录号:JF499694)。
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| 关 键 词： | 八氢番茄红素合成酶 君子兰 克隆 序列分析 |
Cloning and sequence analysis of phytoene synthase cDNA from Clivia miniata Rogel |
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| LAO Shanshan
,
YUAN Zhiming
,
QIN Zhiwei
,
ZHOU Xiuyan.Cloning and sequence analysis of phytoene synthase cDNA from Clivia miniata Rogel[J].Journal of Northeast Agricultural University,2012,43(4):113-117. |
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| Authors: | LAO Shanshan YUAN Zhiming QIN Zhiwei ZHOU Xiuyan |
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| Institution: | (College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | A pair of specific primers was designed according to the PSY mRNA sequene of Narcissus pseudo narcissus(GenBank accession number X78814.1) and a novel 1.4 kb cDNA was obtained from Clivia miniata Rogel by PCR method.The sequence analysis showed that the length of cDNA was 1 395 bp with an open reading frame of 1 272 bp.This gene encoded a polypeptide of 423 animo acids.Its cDNA sequence showed 98%-76% identity with PSY genes from Narcissus tazetta(DQ984674),Oncidium gower Ramsey(FJ859988) and Camellia sinensis(EF545005).Its amino acid sequence shared 99%-80% identity with PSY protein from Narcissus tazetta,Actinidia deliciosa,Solanum lycopersicum and Diospyros kaki,respectively.The results indicated that the sequence obtained in this study was the full length PSY cDNA from Clivia miniata Rogel and the GenBank accession number was JF499694. |
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| Keywords: | phytoene synthase Clivia miniata Rogel cloning sequence analysis |
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