| 马皮肤成纤维细胞的分离与体外培养 |
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| 引用本文： | 刘春霞,余凤玲,周欢敏.马皮肤成纤维细胞的分离与体外培养[J].黑龙江畜牧兽医,2007(6):11-13. |
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| 作者姓名： | 刘春霞 余凤玲 周欢敏 |
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| 作者单位： | 内蒙古农业大学生物工程学院，内蒙古农业大学动物科学与医学学院，内蒙古农业大学生物工程学院 内蒙古 呼和浩特 010018，内蒙古 呼和浩特 010018，内蒙古 呼和浩特 010018 |
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| 基金项目： | 教育部高校骨干教师资助计划 |
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| 摘 要： | 研究用小组织块直接培养法和酶消化法成功地分离了马皮肤成纤维细胞,并对其进行了体外培养,同时筛选出消化马皮肤组织块的适宜酶及酶浓度,获得了马皮肤成纤维细胞的生长曲线,并发现至少在传至第12代时有81.3%的染色体还保持正常的倍数(2n=64)。
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| 关 键 词： | 马 皮肤 成纤维细胞 体外 培养 |
| 文章编号： | 1004-7034（2007）06-0011-03 |
| 收稿时间： | 2006-05-09 |
| 修稿时间： | 2006-05-09 |
Dissociation and culture of horse skin fibroblasts in vitro |
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| LIU Chun-xia,YU Fen-ling,ZHOU Huan-min.Dissociation and culture of horse skin fibroblasts in vitro[J].Heilongjiang Animal Science And veterinary Medicine,2007(6):11-13. |
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| Authors: | LIU Chun-xia YU Fen-ling ZHOU Huan-min |
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| Institution: | 1. College of Biotechnology, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. College of Animal Science and Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China |
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| Abstract: | Horse skin fibroblasts were successfully dissociated and cultured in vitro by explant tissue culture combined with enzyme digestion.Optimal enzyme and concentration of enzyme for digestion of horse skin tissue were screened.The growth curve of horse skin fibroblasts was obtained.Horse skin fibroblast had 81.3% normal chromosome content after at least twelve passages(2n=64). |
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| Keywords: | Horse skin fibroblast in vitro culture |
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