H2S和NO在大白菜抵抗高温胁迫中的作用

结合药理学和植物生理生化的方法,通过外源施加供体或清除剂,并观察幼苗生长的变化,意在研究H_2S和NO两种气体信号分子在大白菜抵抗高温胁迫中的生理作用及相互影响。实验结果表明:高温胁迫明显抑制大白菜幼苗的生长,导致植株叶片明显萎蔫卷曲。胁迫条件下,植株可溶性糖、可溶性蛋白、脯氨酸含量以及相对电导率均显著升高,内源H_2S和NO含量明显上升,外源生理浓度H_2S和NO处理可明显缓解高温胁迫对幼苗生长的影响,并且两种信号分子存在相互作用,外源H_2S缓解内源NO含量的上升,而外源NO对内源H_2S含量变化并没有显著影响。综上,气体信号分子H_2S和NO交叉互作参与大白菜抵御高温胁迫。

Effects of H2S and NO on resistance to high temperature stress in Chinese cabbage

We discussed their interactions and physiological effects on the resistance to high temperature stress in Chinese cabbage using pharmacological and plant biochemical methods. It was found that high temperature stress significantly inhibited the growth of Chinese cabbage seedlings and led to wilting and obvious curling of leaves. The content of soluble sugar, soluble protein, and proline, and endogenous H₂S and NO, as well as the relative conductivity, increased considerably under high temperature stress. Physiological concentrations of exogenous H₂S and NO could notably mitigate the inhibition of high temperature stress on seedling growth; a strong interaction between H₂S and NO was found. Exogenous H₂S can alleviate the formation of endogenous NO, but not vice versa. Overall, H₂S and NO together enhance the response of cabbage to high temperature stress.