| 一起穿山甲疫情的病原分析 |
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| 引用本文： | 陶立,闫鼎羽,李军,马春霞,陈泽祥,梁文汇,阙腾程,曾鹏,李宝财,杨威.一起穿山甲疫情的病原分析[J].动物医学进展,2016(5):133-136. |
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| 作者姓名： | 陶立 闫鼎羽 李军 马春霞 陈泽祥 梁文汇 阙腾程 曾鹏 李宝财 杨威 |
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| 作者单位： | 1. 广西兽医研究所，广西南宁 530001; 广西畜禽疫苗新技术重点实验室，广西南宁 530001;2. 广西林业科学研究院,广西南宁,530002;3. 广西陆生野生动物救护研究与疫源疫病监测中心,广西南宁,530028 |
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| 基金项目： | 国家林业局部门预算广西项目(2015008) |
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| 摘 要： | 为了查明一起人工养殖穿山甲疫情的致病原因,应用病毒PCR检测和细菌分离的方法对2只前后病死穿山甲进行病原学检测。病毒PCR检测结果显示,未能从肺组织中检测到流感病毒、副黏病毒和腺病毒核酸。细菌分离结果表明,从2只病死穿山甲肺中各分离到1株优势菌株。细菌的生化试验、16S rRNA基因的序列分析表明,2株分离菌株均为摩氏摩根菌。小鼠的致病试验和分离菌的药敏试验结果表明,2株分离菌对小鼠有很强的致病作用,对新霉素、壮观霉素、阿米卡星高度敏感。
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| 关 键 词： | 穿山甲 摩氏摩根菌 分离鉴定 药敏试验 |
Pathogenic Analysis of A Disease Epidemic in Pangolins |
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| Abstract: | In order to find out the cause of disease epidemic in raised pangolins,samples from two dead pan-golins were detected by bacterial isolation and PCR.Influenza virus,paramyxovirus or adenovirus virus could not be detected by PCR.Two bacterial strains were isolated from lungs of dead pangolins.Biochemi-cal tests,16 S rRNA sequence analysis of bacteria indicated that both isolates were Morganella morganii . Animal pathogenicity test suggested that the two isolates had strong pathogenic effect;while drug sensitiv-ity test showed that both isolates were highly sensitive to neomycin,spectinomycin and amikacin. |
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| Keywords: | pangolin Morganella morganii isolation drug sensitivity test |
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