金银花灰毡毛忍冬组培快繁技术研究 |
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引用本文: | 李永兴,张丽云.金银花灰毡毛忍冬组培快繁技术研究[J].现代农业科技,2010(12):101-102,106. |
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作者姓名: | 李永兴 张丽云 |
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作者单位: | 湖南生物机电职业技术学院,湖南长沙,410127 |
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基金项目: | 湖南生物机电职业技术学院院级重点课题 |
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摘 要: | 利用金银花灰毡毛忍冬春季的茎段进行组织培养,筛选出了灰毡毛忍冬不同组培阶段适宜的培养基配方,结果认为:在初级培养阶段为A9号(MS+0.10 mg/LBA+0.10 mg/LIBA)培养基,外植体的分化率达92.50%;在继代培养阶段为B8号(MS+1.50 mg/LBA+0.05mg/L NAA)培养基,不仅灰毡毛忍冬苗生长旺盛,而且具有较高的增殖系数,增殖倍数达4倍以上;在生根培养阶段为C3号(1/2MS+1.50mg/L NAA)培养基,试管苗生根率达95%、驯化成活率达92%以上。
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关 键 词: | 金银花 灰毡毛忍冬 茎段 组织培养 |
Research on Tissue Culture and Rapid Propagation of Honeysuckle Lonicera Macranthoides |
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Authors: | LI Yong-xing ZHANG li-yun |
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Institution: | LI Yong-xing ZHANG li-yun(Hunan Biological and Electromechanical Polytechnic,Changsha Hunan 410127) |
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Abstract: | Using stem of honeysuckle Lonicera macranthoides growing in the spring in tissue culture,the appropriate tissue culture medium formula at different stages for Lonicera macranthoides were screened.Results showed that:in the initial training phase with the culture medium A9(MS+ 0.10 mg/LBA+0.10 mg/LIBA),differential ratio of explant reaches 92.50%;in the subculture stage with the culture medium B8(MS+1.50 mg/L BA+0.05 mg/L NAA),not only Lonicera macranthoides spout grews vigorously,but also with high multipli... |
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Keywords: | honeysuckle Lonicera macranthoides stem tissue culture |
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