| 甘蔗品种ROC22组织培养体系中G418的浓度筛选 |
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| 引用本文： | 刘家仪,杨丽涛,李杨瑞.甘蔗品种ROC22组织培养体系中G418的浓度筛选[J].中国甜菜,2014(3):9-11. |
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| 作者姓名： | 刘家仪 杨丽涛 李杨瑞 |
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| 作者单位： | [1]亚热带农业生物资源保护与利用国家重点实验室／广西大学农学院,南宁530005 [2]中国农业科学院甘蔗研究中心／广西农业科学院甘蔗研究所、农业部广西甘蔗生物技术与遗传改良重点实验室／广西甘蔗遗传改良重点实验室,南宁530007 |
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| 基金项目： | 国家863计划课题（2013AA102604）、国家国际合作项目（2013DFA31600）、广西自然科学基金项目（2011GXNSFF018002、2013NXNSFAA019073）、广西八桂学者和特聘专家专项基金. |
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| 摘 要： | 以甘蔗品种ROC22茎尖组织为材料,在组织培养的各个阶段,设定添加G418的浓度梯度进行筛选,找出甘蔗品种ROC22遗传转化体系建立的组织培养每个阶段最佳的G418浓度,为应用遗传转化改良甘蔗品种提供参考依据.研究发现,甘蔗品种ROC22茎尖愈伤组织生长、分化、小苗生根阶段的最佳G418浓度分别为20 mg/L、40mg/L、30mg/L.≤20mg/L浓度的G418能促进甘蔗分化,当G418达到一定浓度甘蔗不能生长,因此G418可用于筛选带有其抗性的植株.
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| 关 键 词： | 甘蔗 组织培养 G418 筛选浓度 |
Screening of G418 Concentrations in Tissue Culture of Sugarcane ROC22 |
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| Authors: | LIU Jia-yi YANG Li-tao LI Yang-rui |
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| Institution: | 1. State Key Laboratory of Subtropical Agro-bioresources Conservation and Utilization / College of Agriculture, Guangxi University, Nanning 530005, China; 2. Sugarcane Research Center, Chinese Academy of Agricultural Sciences /Sugarcane Research Institute, Gaangxi Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Gmmgxi), Ministry of Agriculture /Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China) |
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| Abstract: | The stem tip tissue of sugarcane cultivar ROC22 was employed for tissue culture, and ditterent C418 （geneticin） concentration gradients were added in the media to screen optimal concentrations of G418 at different stages of tissue culture and to provide a reference for further genetic improvement through biotechnology. The results showed that the best G418 concentration for callus growth, differentiation and rooting was 20 mg/L, 40 rag/ L and 30 mg/L, respectively. G418 concentration ≤20 mg/L promoted the differentiation. Sugarcane plantlets could not grow when the G418 concentration was upto a certain value, and it could be used for screening the resistant plantlets. |
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| Keywords: | sugarcane tissue culture geneticin （G418） screening concentration |
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