甜菜干种子DNA提取的不同方法比较

分别采用SDS法、CTAB法及碱裂解法对甜菜干种子基因组DNA进行提取．并利用1％琼脂糖判断DNA的纯度，λDNA判断DNA的含量，结果表明，SDS法和CTAB法提取的甜菜基因组DNA含量较高，提取的DNA总量接近，碱裂解法提取的DNA含量较低。3种方法提取的DNA均能够满足SSR—PCR的要求，并且扩增务带没有差异，由于碱裂解法提取DNA快速和简单，因此适合大群体DNA的提取及对模板要求不高的PCR反应中，而SDS法及CTAB法适合一次性大量提取甜菜基因组DNA以及对于DNA纯度要求较高的PCR反应中。

Different Methods of DNA Extraction from Dry Beet Seeds

Abstract：The genomic DNA of dry beet seeds of was extracted by SDS, CTAB or alkaline lysis method, and used 1% agarose to determine the purity of DNA and hDNA to judge the content of DNA. The results showed that the content of genomic DNA by adopting SDS or CTAB was higher, and both the DNA extracts were close to the total amount. The content of DNA was lower by using alkaline lysis method. However, the DNA extracted by the all three approaches could meet the requirements of SSR-PCR, without any difference in amplification bands. As it was fast and simple to extract DNA by alkaline lysis method, it would be suitable for extracting massive DNA as well as the PCR reaction without a high demand for the template. However, both SDS and CTAB are fit for an one-off massive extraction of the genomic DNA of beets, and for the PCR reaction with a high demand of the purity DNA.