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甜菜褐斑病抗感种质细胞超微结构变化比较
引用本文:丁广洲,李永刚,赵春雷,王希,陈丽,马凤鸣,陈连江.甜菜褐斑病抗感种质细胞超微结构变化比较[J].中国甜菜,2014(1):1-4.
作者姓名:丁广洲  李永刚  赵春雷  王希  陈丽  马凤鸣  陈连江
作者单位:[1]黑龙江省普通高等学校甜菜遗传育种重点实验室,哈尔滨150080 [2]中国农业科学院甜菜研究所,哈尔滨150080 [3]东北农业大学农学院,哈尔滨150030
基金项目:中国博士后科学基金项目(2012M520781)资助;国家甜菜产业技术体系建设项目(CARS-210101)资助.感谢国家甜菜中期库对本文部分试验提供材料!
摘    要:在褐斑病发病严重地块。对发病盛期甜菜抗感病种质资源叶片X光片影像及电镜显微结构进行了比较和分析,结果表明:感病种质叶片图像密度不均,整体影像出现泡状光影。受病原茵的侵蚀,感病种质表现为细胞壁变形、并发生质壁分离;叶绿体变形、片层结构零乱、有的膨胀扭曲并分解;线粒体膜破损,膜局部破裂,内舍物外流。出现大量的嗜锇颗粒,有的细胞器逐渐解体、膜系统遭到严重破坏,甚至整个细胞死亡。但抗病种质却明显不同,X衍射图像密度均匀.细胞结构相对完整。

关 键 词:甜菜褐斑病  抗感病种质  叶片细胞  超微结构变化

Comparison of Cellular Ultrastructure of Different Sugarbeet Germplasms Infected Cercospora betico
Authors:DING Guang-zhout  LI Yong-gang  ZHAO Chun-lei  WANG Xi  CHEN Li  MA Feng-ming  CHEN Lian-jiang
Institution:1. Key Laboratory of Sugarbeet Genetic Breedingcqteilongjiang University, Harbin 150080, China; 2. Sugarbeet Research Institute of Chinese Academy Agricultural Sciences, Harbin 150080, China; 3. College of Agriculture, Northeast Agricultural UnbJersity, Harbin 150030, China)
Abstract:The X-ray image and uhrastructure change susceptible germplasm of sugarbeet in cercospora leaf spot that X-ray image density of susceptible germplasm leaf was of foliar cell were compared between resistant and nursery of continuous cultivation. The results showed not uniform, and the X-ray image presented bubbles, cytoderm infected by cercospora was partly deformed and cytoplasm was shrunk away from the cytoderm in the susceptible germplasm; chloroplast was distorted, lamellar structure was disordered, even some were inflated and decomposed; mitochondrial membrane was damaged and inclusion was overflowed. Osmiopilic globules increased obviously; organelle disintegrated gradually, membrane system was seriously destroyed, even entire cell was dead. But those of resistant germplasm were significantly different; the density of picture was even, cell structure was relatively intact.
Keywords:cercospora leaf spot  resistant and susceptible germplasm  foliar cell  uhrastructure change
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