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猪宰后肌肉中钙激活蛋白酶活性变化的研究
引用本文:马俪珍,王永辉,范三红.猪宰后肌肉中钙激活蛋白酶活性变化的研究[J].农业工程学报,2007,23(2):239-243.
作者姓名:马俪珍  王永辉  范三红
作者单位:1. 天津农学院食品科学系,天津,300384
2. 山西农业科学旱地农业研究中心,太原,030006
3. 山西大学生命科学学院,太原,030006
基金项目:山西农业大学校科研和教改项目
摘    要:该文研究杂种野猪和本地白猪屠宰后,肌肉中钙激活蛋白酶系的活性变化,同时,对肌肉的全蛋白提取液作SDS-PAGE分析。结果表明:杂种野猪肉的钙激活蛋白酶活性较高,但表现酶活性的自溶率变化在早期较慢;电泳图谱可看到,杂种野猪蛋白降解较慢,在宰后冷藏40 h的电泳图可看到小分子物质降解较少。

关 键 词:杂种野猪  钙激活蛋白酶系  聚丙烯酰胺凝胶电泳(SDS-PAGE)
文章编号:1002-6819(2007)2-0239-05
收稿时间:2005/12/31 0:00:00
修稿时间:2005-12-31

Changes of calpain activity in postmortem meat of pig
Ma Lizhen,Wang Yonghui and Fan Sanhong.Changes of calpain activity in postmortem meat of pig[J].Transactions of the Chinese Society of Agricultural Engineering,2007,23(2):239-243.
Authors:Ma Lizhen  Wang Yonghui and Fan Sanhong
Institution:Food Science Department, Tianjin Agricultural College, Tianjin 300384, China;Arid farming reseach center,Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;School of Life Scicnce and Technology, Shanxi University, Taiyuan 030006, China
Abstract:Calpain activity changes of wild hybrid pig and home white pig were investigated, and SDS-PAGE analyzsis on extracting solutions of whole protein in the meat was done. Results indicate that the crossbred wild pigs' activities of calpains is more higher, however, the rate of autolysis expressing the activity of calpains at the early stage is very slower; The map of SDS-PAGE photographs show that proteolysis of crossbred wild pig is slower than that of home white pig. Also its small molecular material degraded comparatively slower from SDS-PAGE map after 40-hour cold storage.
Keywords:wild hybrid pig  calpains  SDS-PAGE
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