| 用RT-PCR检测PRSV在番木瓜体内的运转 |
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| 引用本文： | 麦丽芳,黄建昌,肖艳.用RT-PCR检测PRSV在番木瓜体内的运转[J].福建果树,2008(4). |
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| 作者姓名： | 麦丽芳 黄建昌 肖艳 |
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| 作者单位： | 1. 广东省云浮市云城区农业技术推广中心,广东,云浮,527300
2. 仲恺农业工程学院农业与园林学院 |
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| 基金项目： | 广东省科技计划项目
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| 摘 要： | 以抗病突变体岭抗1号与穗中红番木瓜为试材,人工接种PRSV-Ys株系,应用RT-PCR技术检测PRSV在体内的运转.结果表明,感病品种穗中红接种后24 h,即可在接种叶的未接种部位检出病毒,第10 d在接种植株的各部位均可检出病毒;而在岭抗1号植株上,接种后直至第10 d,仍未能在其他部位检测出病毒,表明岭抗1号具有阻碍病毒运转的能力.
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| 关 键 词： | 番木瓜 |
Detection of PRSV Movement in Papaya with RT-PCR |
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| MAI Li-fang,HUANG Jian-chang,XIAO Yan.Detection of PRSV Movement in Papaya with RT-PCR[J].Fujian Fruits,2008(4). |
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| Authors: | MAI Li-fang HUANG Jian-chang XIAO Yan |
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| Institution: | MAI Li-fang1 HUANG Jian-chang2 XIAO Yan2(1Yunfu Agricultural Technology Promotion Centre,Yunfu 527300,Guangdong China,2Department of Agriculture , garden,Zhongkai University of Agriculture , Engineering,Guangzhou 510225,Guangdong China) |
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| Abstract: | RT-PCR was used to detect PRSV movement in resistance to PRSV of a natural mutant(LK-1).The results showed that PRSV was detected in the other parts from inoculated site in the leaves of susceptible plants(suizhonghong) after 24 hour inoculation,and 10 days in all parts of plants.However,PRSV could not be detected in the other parts of inoculated site in the leaves of resistant plants(LK-1).it was proposed that resistance to PRSV could prevent the virus from moving out of inoculated sites or/and into non-in... |
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| Keywords: | PRSV RT-PCR |
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