Validierung der Extraktion von Nematodenzysten aus Bodenproben mit dem MEKU-Bodenextraktor

The diagnosis of phytopathogenic agents has to be justiciable. Quality management gains increasing importance. In preparation of accreditation of the diagnostic laboratory at the Agricultural Public Service Center Rheinhessen-Nahe-Hunsrück in Bad Kreuznach the extraction of cyst nematodes from soil samples by means of a MEKU-soil extractor was validated according to the principles of the German Accreditation Association (DAkkS). The results achieved with test procedure were analyzed according to the parameters analytical sensitivity, analytical specification, selectivity, repeatability and reproducibility. The limit of acceptance kept the conditions of the quality control plan. Therefore the test procedure is qualified for diagnosis of quarantine organisms according to the specifications of DAkkS. Continuous participation in laboratory comparative tests ensures a long term, high level quality standard.