| 葡萄AFLP技术体系建立及其在超藤与藤稔葡萄品种鉴别中的应用 |
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| 引用本文： | 鲍露,徐昌杰,江文彬,陈履荣,陈昆松.葡萄AFLP技术体系建立及其在超藤与藤稔葡萄品种鉴别中的应用[J].果树学报,2005,22(4):422-425. |
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| 作者姓名： | 鲍露 徐昌杰 江文彬 陈履荣 陈昆松 |
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| 作者单位： | 1. 浙江大学园艺系,农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029
2. 浙江省金华市婺江葡萄研究所,浙江,金华,321017 |
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| 摘 要： | 超藤葡萄是藤稔葡萄的一个芽变品种,由于2者亲缘关系十分接近,应用形态学手段以及RAPD分子标记不易区分。针对葡萄叶片富含多糖和多酚类等杂质的特点,从基因组DNA纯化入手,对AFLP流程进行了优化改进,建立了葡萄AFLP体系,并成功地对此2个品种进行了区分。采用引物组合M-CAT+E-ACG进行AFLP扩增,超藤葡萄出现167bp特异带。
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| 关 键 词： | 葡萄 AFLP 品种鉴别 |
| 文章编号： | 1009-9980(2005)04-422-04 |
| 修稿时间： | 2004年10月12 |
Establishment of an AFLP protocol for grape and cultivar distinguishing of chaoteng and Fujiminori |
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| BAO Lu,XU Chang-jie,JIANG Wen-bin,CHEN Lu-rong,CHEN Kun-song.Establishment of an AFLP protocol for grape and cultivar distinguishing of chaoteng and Fujiminori[J].Journal of Fruit Science,2005,22(4):422-425. |
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| Authors: | BAO Lu XU Chang-jie JIANG Wen-bin CHEN Lu-rong CHEN Kun-song |
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| Abstract: | Chaoteng grape, as a somatic mutant of Fujiminori, is quite difficult to be distinguished from its parental line by morphological or even RAPD methods. Here we provided an improved AFLP protocol suitable for grape whose leaves are highly rich in impurities including polysaccharides and polyphenols. And the two grape cultivars were successfully discriminated when AFLP primers M-CAT and E-ACG were applied,where Chaoteng produced a specific AFLP product of 167 bp in length. |
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| Keywords: | Grape (Vitis vinifera L ) AFLP Cultivar distinguishing |
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