| 伊氏锥虫同工酶、蛋白质和抗原组分的比较研究 |
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| 引用本文： | 王云飞,沈杰,郑韧坚.伊氏锥虫同工酶、蛋白质和抗原组分的比较研究[J].畜牧兽医学报,1993(5). |
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| 作者姓名： | 王云飞 沈杰 郑韧坚 |
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| 作者单位： | 中国农业科学院上海家畜寄生虫病研究所 200232
(王云飞，沈杰)，中国农业科学院上海家畜寄生虫病研究所 200232(郑韧坚) |
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| 基金项目： | 国家科学基金资助项目,编号38970579 |
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| 摘 要： | 本文采用生化技术对八个中国伊氏锥虫株及一个布氏锥虫株的同工酶、蛋白质和抗原组分进行比较研究。根据同工酶电泳结果,可将伊氏锥虫和与其形态上不能区分的布氏锥虫区别开来,亦可将伊氏锥虫分为两个酶株群(Z1、Z2)。根据SDS-聚丙烯酰胺凝胶电泳、等电聚焦电泳和免疫印迹试验的结果,可将酶株群Z1分成五个不同多肽群(株)。本研究结果表明,中国伊氏锥虫遗传变异程度较低,是一个相对稳定的种群。
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| 关 键 词： | 同工酶 酶株群 多肽群 伊氏锥虫 布氏锥虫 |
A COMPARISON OF THE ISOENZYMES,PROTEINS AND ANTIGENS OF TRYPANOSOMA EVANSI |
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| Wang Yunfei,Shen Jie,Zheng Renjian.A COMPARISON OF THE ISOENZYMES,PROTEINS AND ANTIGENS OF TRYPANOSOMA EVANSI[J].Acta Veterinaria et Zootechnica Sinica,1993(5). |
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| Authors: | Wang Yunfei Shen Jie Zheng Renjian |
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| Abstract: | Characterization of enzymes, proteins and antigens of 8 Trypanosoma evansi strains isolated in China and 1 Trypanosoma brucei strain was studied by biochemical techniques. By isoenzyme electro-phoresis, we distinguished all the T. evansi strains from the T. brucei strain undistinguished by morphological study, and also divided the T. evansi strains into 2 zymodemes (Zl, Z2). By sodium dodecylsulph-polyacrylamide gel electro-phoresis, isoelectric focusing and western-blotting, we subdivided Zl into 5 peptidemes. This study showed that the T. evansi isolated in China had low genetic variation, and formed a relatively stable spiecies. |
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| Keywords: | Isoenzyme Zymodemes Peptidemes Trypanosoma evansi Trypano-soma brucei |
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