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| 近交五指山小型猪精液冷冻保存技术的研究 | |
| 引用本文： | 崔茂盛,敖红,张莉,叶邵辉,冯书堂.近交五指山小型猪精液冷冻保存技术的研究[J].中国畜牧杂志,2007,43(3):11-14. |
| 作者姓名： | 崔茂盛 敖红 张莉 叶邵辉 冯书堂 |
| 作者单位： | 1. 中国农业科学院畜牧研究所,北京,100094;云南农业大学动物科技学院,云南昆明,650201 2. 中国农业科学院畜牧研究所,北京,100094 3. 云南农业大学动物科技学院,云南昆明,650201 |
| 基金项目： | 科技部中国实验用小型猪资源开发与研究应用项目;基础性工作和社会公益研究专项课题 |
| 摘 要： | 用液氮熏蒸法在氟板上制作冻精颗粒,以解冻后的精子活率、活力和质膜完整性为判定指标,比较4种冷冻稀释液及不同冷冻-解冻程序对五指山小型猪精液冷冻的效果。结果表明:①Ⅳ号冷冻稀释液冷冻解冻后精子的活率(0.610±0.036)、活力(0.427±0.025)和质膜完整性(0.503±0.015)均显著高于Ⅰ、Ⅱ和Ⅲ号冷冻稀释液(P<0.05)。②实验中精液在4℃冰箱中平衡降温2 h的精液精子活力、质膜完整性均好于在17℃平衡3 h再放入4℃冰箱中平衡2 h的解冻效果,而且精子活率差异显著(P<0.05)。③湿解法的效果优于干解法。 |
| 关 键 词： | 精液冷冻 活力 质膜完整率 五指山小型猪 |
| 文章编号： | 0258-7033(2007)03-0011-03 |
| 修稿时间： | 2005-11-16 |
Study on WZSP Semen Cryopreservation Techniques |
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| CUI Mao-sheng,AO Hong,ZHANG Li,YE Shao-hui,FENG Shu-tang.Study on WZSP Semen Cryopreservation Techniques[J].Chinese Journal of Animal Science,2007,43(3):11-14. | |
| Authors: | CUI Mao-sheng AO Hong ZHANG Li YE Shao-hui FENG Shu-tang |
| Institution: | 1.Institute of Animal Science, Chinese Academy of Agriultural Sciences, Beijing 100094, China; 2.College of Animal Science and Technology, Yunnan Agricultural University, Yunnan Kunming 650201, China |
| Abstract: | Frozen boar semen pellets were made on the fluorin plant.To select out a best freezing extender from several extenders and freezing-thawing protocols,sperm viability,motility and plasma membrane integrity were studied in this experiment.The results showed that①the number Ⅳ extender was the best among the extenders tested and its sperm motility(0.427±0.025),viability(0.610±0.036) and plasma membrane integrity(0.503±0.015) after-thawing were all significantly higher than those obtained with number Ⅰ、Ⅱ and Ⅲ extenders(P<0.05);② The sperm motility and plasma membrane integrity of semen cooling for 2 h at 4℃ after-thawing were both better than those cooling for 3 h at 17℃ and then transferring in 4℃ refrigerator for 2 h.And the sperm viability had significant differences(P<0.05);③The effects of thawing in solution were better than those out of dried thawing. |
| Keywords: | semen cryopreservation motility plasm membrane integrity WZSP |
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