| 黄金鲈鱼的一种ZP基因的电子克隆与鉴定分析 |
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| 引用本文： | 孙业盈,单长民.黄金鲈鱼的一种ZP基因的电子克隆与鉴定分析[J].安徽农业科学,2009,37(24):11456-11458. |
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| 作者姓名： | 孙业盈 单长民 |
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| 作者单位： | 滨州医学院基础学院,山东烟台,264003;滨州医学院基础学院,山东烟台,264003 |
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| 基金项目： | 滨州医学院科研启动基金 |
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| 摘 要： | 目的]克隆分析黄金鲈鱼的一种邪基因。方法]利用生物信息学手段克隆了黄金鲈鱼的一种ZP基因的全长CDNA序列，并对其序列进行了分析。结果]该cDNA序列包含1653bp的开放阅读框，编码550个氨基酸。氨基酸序列同源性分析和进化分析表明，黄金鲈鱼的ZP蛋白与丰滑舌鳎ZP3b和青鳝ZPC5具有较高的同源性。结论]所获得的黄金鲈鱼的ZP基因属于ZP3类型。
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| 关 键 词： | 黄金鲈鱼 ZP基因 电子克隆 生物信息学 |
Silico Cloning and Analysis of ZP Gene in Yellow Perch |
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| SUN Ye-ying et al.Silico Cloning and Analysis of ZP Gene in Yellow Perch[J].Journal of Anhui Agricultural Sciences,2009,37(24):11456-11458. |
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| Authors: | SUN Ye-ying |
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| Institution: | SUN Ye-ying et al(Basic College,Binzhou Medical University,Yantai,Sh,ong 264003) |
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| Abstract: | Objective] One ZP gene of yellow perch was cloned and analyzed.Method] The ZP gene of yellow perch was obtained and analysed by bioinformatics method.Result] The sequence of the gene contained 1 653 bp open reading frame(ORF) and encoded 550 amino acids.Alignment analysis and molecular evolution analysis showed that the ZP protein was much similar with ZP3b of half-smooth tongue sole and ZPC5 of medaka.Conclusion] The ZP gene of yellow perch belongs to the type of ZP3. |
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| Keywords: | Yellow perch ZP gene Silico clone Bioinformatic |
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