马铃薯根组织培养与植株再生

利用马铃薯温室生长幼芽和组培根组织进行了以MS为培养基、附加不同种类和浓度生长素和细胞分裂素组成的8种培养基,在散光和黑暗条件下培养诱导愈伤组织,在3种分化培养基上培养分化再生植株的试验。结果表明,青薯5号和青薯8号温室生长幼芽根组织接种MS＋2,4-D 2mg/L＋BAP 3mg/L＋NAA 0.5mg/L＋蔗糖30g/L＋琼脂7g/L培养基上散光条件下培养45-90d产生愈伤组织,诱导率为0.024%;青薯5号愈伤组织接种1/2MS＋2,4-D 0.5mg/L＋BAP 2mg/L＋KT 2mg/L＋GA30.5mg/L＋ZT0.2mg/L＋蔗糖20g/L＋琼脂7g/L培养基上培养80d分化出再生植株,分化率为6.25%,再生植株切成一叶茎段接种于MS＋BAP 0.1mg/L＋IAA 10mg/L＋GA30.1mg/L＋白糖30g/L＋琼脂6g/L培养基上培养3周长成株高8-10 cm、叶7-10片、叶色深绿和根系发达的小植株。

Study on Plantlet Production from Cultured Root Tissue of Potato

Root segments both from tissue culture plantlets and buds in greenhouse cultured of potato cultivars： Qingshu No.5 and Qingshu No.8 were used to study induction callus and organ regeneration.The results showed that callus started forming at root segments from buds of potato cultivar： Ingush No.5 and Ingush No.8 in greenhouse cultured 45d after placement on culture medium： MS＋2,4-D 2mg/L＋BAP 3mg/L＋NAA 0.5mg/L＋sucrose 30g/L＋agar 7g/L and diffused light.The frequency of callus induction was 0.024%.Shoot developed from callus of cuhivar Qingshu No. 5 80d after cultured on differentiation medium：1/2MS ＋2,4 - D 0.5mg/L ＋ BAP 2mg/L ＋ KT 2mg/L ＋ GA3 0. 5mg/L ＋ ZT 0. 2mg/L ＋ sucrose 20g/L ＋ agar 7g/L. The frequency of shoot regeneration was 6. 25%. Plantlets developed from shoot 3 weeks .after cultured on rooting medium： MS ＋ BAP 0. lmg/L ＋ IAA 10mg/L ＋ GA3 0. lmg/L ＋ surose 30g/L ＋ agar 6g/L.