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| 白平菇细胞工程菌株的耐热性研究 | |
| 引用本文： | 王谦,齐玲娣,付强,李志. 白平菇细胞工程菌株的耐热性研究[J]. 安徽农业科学, 2007, 35(5): 1364-1364,1370. DOI: 10.3969/j.issn.0517-6611.2007.05.048 |
| 作者姓名： | 王谦 齐玲娣 付强 李志 |
| 作者单位： | 河北大学生命科学学院,河北保定,071002;河北大学生命科学学院,河北保定,071002;河北大学生命科学学院,河北保定,071002;河北大学生命科学学院,河北保定,071002 |
| 基金项目： | 基金项目,河北省科技厅项目（04230132D）;河北省生物工程技术研究中心项目资助. |
| 摘 要： | 将通过原生质体再生技术制备的19株白平菇细胞工程菌株,分别在20、25、30和35 ℃中培养.结果表明,有6株细胞工程菌株表现出了比出发菌株较高的耐热能力,同时,所有的菌株在20、25和30 ℃下均能生长,而在35℃条件下菌丝只能萌发,不能生长.经进一步的耐热性菌株的筛选,选育出了一株较耐热的菌株P484. |
| 关 键 词： | 白平菇 细胞工程菌株 耐热性 |
| 文章编号： | 0517-6611（2007）05-01364-01 |
| 修稿时间： | 2006-11-06 |
Study on the Thermotoleranee of Pleurotus ostreatus Cell Engineering Strains |
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| WANG Qian et al. Study on the Thermotoleranee of Pleurotus ostreatus Cell Engineering Strains[J]. Journal of Anhui Agricultural Sciences, 2007, 35(5): 1364-1364,1370. DOI: 10.3969/j.issn.0517-6611.2007.05.048 | |
| Authors: | WANG Qian et al |
| Affiliation: | College of Life Science, Hebei University, Baoding, Hebei 071002 |
| Abstract: | 19 cell engineering strains regenerated by protoplast from Pleurotus ostreatus were studied.When these strains were cultivated under 20,25,30 and 35 ℃,it was found out that six regenerative strains of protoplast grew faster than the original strain.Meanwhile,all the mycelium could grow at 20,25 and 30 ℃,but at 35 ℃ the mycelium could only bourgeon and was unable to grow.The result of further high-temperature-tolerant test showed that the strain P484 was more heat-resistant than other strains.This test proved the feasibility of breeding on edible fungi by the technology of protoplast regeneration. |
| Keywords: | Pleurotus ostreatus Cell engineering strains Thermotolerance |
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