| 红树叶蛋白质样品制备方法的比较及其双向电泳分析 |
| |
| 引用本文： | 彭存智,李蕾,刘志昕.红树叶蛋白质样品制备方法的比较及其双向电泳分析[J].热带生物学报,2010,1(1):12-16. |
| |
| 作者姓名： | 彭存智 李蕾 刘志昕 |
| |
| 作者单位： | 彭存智,刘志昕(中国热带农业科学院,热带生物技术研究所,农业部转基因植物及植物用微生物环境安全监督检验测试中心,海南,海口,571101);李蕾(海南师范大学,生命科学学院,海南,海口,571101) |
| |
| 基金项目： | 中国热带农业科学院中央公益性科研院所基本科研业务费专项支助 |
| |
| 摘 要： | 采用水溶液提取法(磷酸盐缓冲液系统)、三氯乙酸-丙酮沉淀法和Phenol改良提取法,对红树叶进行蛋白质提取,并比较分析了这3种方法的蛋白质提取率、完整性、溶解性等。结果表明:水溶液提取法得到的是胶状沉淀,基本为多糖类物质,蛋白质含量很低;三氯乙酸-丙酮沉淀法提取的蛋白质纯度较高,但双向电泳背景最低,提取率较低,蛋白质的完整性差;Phenol改良提取法的蛋白质提取率高,约为三氯乙酸-丙酮法的10倍,双向电泳凝胶背景比三氯乙酸-丙酮沉淀法要深,蛋白质的完整性好,能够被电泳分离的蛋白质点是三氯乙酸-丙酮沉淀法的7倍左右。Phenol改良提取法在蛋白质的提取率以及完整性方面远远高于其他2种蛋白质提取方法。
|
| 关 键 词： | 红树 蛋白质提取 双向电泳 |
Comparison and Analysis of Protein Extraction from Rhizophora apiculata B1 |
| |
| PENG Cun-zhi ,LI Lei,LIU Zi-xin.Comparison and Analysis of Protein Extraction from Rhizophora apiculata B1[J].Journal of Tropical Biology,2010,1(1):12-16. |
| |
| Authors: | PENG Cun-zhi LI Lei LIU Zi-xin |
| |
| Institution: | PENG Cun-zhi 1,LI Lei2,LIU Zi-xin1 (1. Institute of Tropical Bioscience and Biotechnology,Environmental Safety Supervision and Inspection Centre for Genetically Modified Plants and Microorganisms used in Plants,Ministry of Agriculture,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101 China,2. Department of Biology,Hainan Normal University,Haikou 571158,China) |
| |
| Abstract: | In order to extract high quality protein sample for two dimensional electrophoresis (2-DE) assay,aqueous solution method,trichloroacetic acid-aceton method and the advanced phenol isolated method were used to extract the proteins from Rhizophora apiculata Bl,and the harvest rate,the integrality and the solubility of the three methods were compared and analyzed. The results showed that the colloidal deposition,which was composed of polysaccharide but little of protein,was isolated by the aqueous solution met... |
| |
| Keywords: | Rhizophora apiculata Bl protein extraction two-dimensional electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
