杭白芷种质资源遗传多样性的AFLP分析

通过对50份杭白芷种质资源的AFLP分析，探求各种质间的遗传多样性。采用扩增片段长度多态性 （AFLP）标记，在DNA水平上进行遗传多样性研究，筛选出12对选择性扩增引物，将扩增出的条带作为原始矩阵，用NTSYS PC软件计算并分析了杭白芷种质间的相似度，构建了遗传系统进化树。结果表明，50份杭白芷种质间，相似度均超过70%，总体上亲缘关系都比较接近，相对而言，ZYS002039和ZYS002045种质，与其他种质的遗传背景差异较大。

AFLP analysis of genetic diversity of Angelica dahurica

The 50 germplasmic resources of Angelica dahurica were analyzed by AFLP to determine the genetic diversity. The amplified fragment length polymorphism （AFLP） tags were used to investigate the genetic diversity at the DNA level. Twelve pairs of selective amplification primers were screened out to amplify the bands， which were used as original matrix and analyzed the similarity between different Angelica dahurica germplasm by NTSYS|PC software calculation and constructed the phylogenetic tree. The results indicated that the similarity of the 50 Angelica dahurica germplasm resources all more than 70%， whose overall phylogenetic relationships were relatively close. The germplasm ZYS002039 and ZYS002045 showed larger difference in genetic background from the others．