部分板栗品种遗传多样性的AFLP分析

利用荧光标记AFLP技术，采用7对M+3和E+3引物组合对30份板栗和日本栗栽培品种进行了总基因组DNA水平上的多态性检测，共获得962条可统计的条带，其中852条呈多态性，多态性带百分率达89％。揭示了板栗丰富的遗传多样性。7组引物在30个品种中检测到数目不等的品种特异带型，对供试板栗品种具有一定的鉴别价值。7对引物能将30个板栗和日本栗品种完全区分开。聚类分析结果表明，多数来源地相同的板栗品种资源表现出较为密切的亲缘关系。

Studies on the Genetic Diversity of Some Chestnut (Castanea) Cultivars by AFLP Analysis

Investigation of the genetic diversity of chestnut (Castanea) is significate in utilizing its germplasm resources and breeding new varieties. The phylogenetic relationships among 30 chestnut cultivars were analyzed by using of AFLP technique to study the genetic diversity with seven AFLP primer combinations (M+3 and E+3). Among the 962 bands detected, 852 bands were polymorphic and specific bands had been found in all tested cultivars. The high ratio of polymorphic bands (89%) observed indicated abundant genetic diversity among chestnut cultivars. The results suggested that the seven pairs of primer combinations could be used to distinguish all tested cultivars. Cluster analysis showed that the classification based on AFLP markers of most cultivars was consistent with their origin.