Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum L.) breeding programmes |
| |
Authors: | Csaba Lantos Jens Weyen José M Orsini Heike Gnad Bernd Schlieter Volker Lein Stefan Kontowski Andreas Jacobi Róbert MihÁly Sue Broughton János Pauk |
| |
Institution: | 1. Department of Biotechnology, Cereal Research Non‐profit Limited Company, , P.O. Box 391 H‐6701 Szeged, Hungary;2. Saaten‐Union Biotec GmbH, , 33818 Leopoldsh?he, Germany;3. Saaten‐Union Biotec GmbH, Subsidiary Gatersleben, Biotechpark Gatersleben, , 06466 Stadt Seeland OT Gatersleben, Germany;4. Deutsche Saatveredelung AG, Saatzucht Leutewitz, , 01665 K?bschütztal, Germany;5. Saaten Union Recherche SARL, , 60190 Estreès‐Saint‐Denis, France;6. W. von Borries‐Eckendorf GmbH & Co. KG, , 33818 Leopoldsh?he, Germany;7. Strube Research GmbH & Co. KG, , 38387 S?llingen, Germany;8. Department of Agriculture and Food WA, , SouthPerth, 6151 WA, Australia |
| |
Abstract: | The efficiency of our anther culture protocol was tested with high‐ and low‐responding genotypes, ‘Svilena’ and ‘Berengar’, and 93 F1 winter wheat crosses in 2010 and 2011. Based on data for these genotypes, the effect of genotype influenced the number of embryo‐like structures, regenerated plantlets and green plantlets, while the number of albino plantlets was affected by genotype, year and environmental factors. Although genotype also influenced the production of green plantlets from breeding crosses, with green plantlets per 100 anthers ranging from 0.04 to 28.67, the average regeneration rate over all crosses was 5.3 green plantlets/100 anthers, which resulted in a total of 11 416 well‐rooted green plantlets. The survival rate of green plantlets following acclimatization was 97.21% in 2010 and 96.34% in 2011. In this study, the phenomenon of albinism and genotype dependency did not hinder the production of more than five thousand green plantlets each year. In our experiments, anther culture proved to be an efficient method in winter wheat breeding programmes with lower costs than alternative technologies. |
| |
Keywords: | anther culture breeding doubled haploid Triticum aestivum L wheat |
|
|