冰草EST-SSR引物和小麦EST-SSR引物在黑麦属基因组的通用性分析

为研究黑麦属植物的遗传多样性,开发R基因组特有的分子标记并绘制其遗传连锁图谱,选用1 343对冰草EST-SSR引物和786对小麦EST-SSR引物对新疆杂草黑麦和栽培黑麦(共计6份材料)的全基因组进行了PCR扩增,结果显示,有679对冰草EST-SSR引物能够扩出清晰的条带,占引物总数的50.6%;其中有187对引物在6份黑麦材料基因组中扩增产物表现为多态性,占其引物总数的13.9%,平均每对引物扩增条带数为1.1。有364对小麦EST-SSR引物可扩增出清晰的条带,占其引物总数的46.3%;其中有135对引物在6份黑麦材料基因组中扩增产物具有多态性,占其引物总数的17.1%,平均每对引物扩增条带数为2.0。冰草EST-SSR引物在黑麦中的有效扩增效率高于小麦EST-SSR的有效扩增效率,但扩增多态性后者大于前者。两种来源引物扩增强带比率分别为51.8%和32.6%。结果表明小麦和冰草的EST-SSR引物均可用于黑麦基因组分析研究。

Analysis of Transferability of Agropyron cristatum L. EST SSR and Wheat EST SSR Primers to Secale L.

In order to study the genetic diversity of Secale L., develop the unique molecular markers and draw a genetic linkage map. 1343 pairs of EST SSR primers of Agropyron cristatum L. and 786 pairs of EST SSR primers of wheat were used to amplify the genomic DNA of 6 materials of S.cereale subsp. segetale and S.cereale L. It was revealed that 679(50.6%) pairs of EST SSR primers of Agropyron cristatum L. brought detectable amplification bands, 187(13.9%) pairs of primers displayed polymorphisms and every effective amplification primer could amplify 1.1 bands on average. There were 346(46.3%) pairs of EST SSR primers of wheat could amplify clear bands, the polymorphism primers were 135(17.1%), and an average of 2.0 bands was amplified for each effective amplification primers. The ratio of effictive amplification of A. cristatum EST SSR primers was more than that of wheat EST SSR primers, but the polymorphism was higher in wheat EST SSR primers. The strong bands of these two sources of materials were 51.8% and 32.6% respectively. The results showed potential for using two sources of primersto analyse the Secale L. genome.