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EMS对玉米花粉诱变效应的研究
引用本文:祝丽英,池书敏,刘志增,孟义江,陈景堂,宋占权,王静华. EMS对玉米花粉诱变效应的研究[J]. 河北农业大学学报, 2002, 25(1): 17-20,28
作者姓名:祝丽英  池书敏  刘志增  孟义江  陈景堂  宋占权  王静华
作者单位:河北农业大学农学院,河北保定071001;河北保定师范专科学校,河北保定,071051
基金项目:河北省自然科学基金资助项目 (30 2 2 83),河北省重大科技攻关项目 (95 - 98- 0 1- 0 1)
摘    要:本试验用含有EMS的石蜡油溶液处理10个玉米材料的成熟花粉,研究了EMS对花粉离体萌发率、M1代生理损伤以及M2代的诱变效应。结果表明:花粉离体萌发率随EMS体积分数的增加而降低,并与M1结实率呈高度正相关;M1受到严重的生理损伤,表现为出苗率低,出苗时间长,成株率下降;M2代中,子粒突变性状以粒色突变为主,苗期突变性状主要是叶片颜色突变,成株期突变性状有株高、株型等多方面;同一处理浓度下,花了体萌发率、M1代生理损伤和M2代的诱变效应的大小因材料品种不同而不同。

关 键 词:玉米  花粉  甲基磺酸乙酯  诱变效应
文章编号:1000-1573(2002)01-0017-04

A study on the effects of EMS on maize pollens
ZHU Li ying ,CHI Shu min ,LIU Zhi zeng ,MENG Yi jiang ,CHEN Jing tang ,SONG Zhan quan ,WANG Jing hua. A study on the effects of EMS on maize pollens[J]. Journal of Agricultural University of Hebei, 2002, 25(1): 17-20,28
Authors:ZHU Li ying   CHI Shu min   LIU Zhi zeng   MENG Yi jiang   CHEN Jing tang   SONG Zhan quan   WANG Jing hua
Affiliation:ZHU Li ying 1,CHI Shu min 1,LIU Zhi zeng 1,MENG Yi jiang 1,CHEN Jing tang 1,SONG Zhan quan 2,WANG Jing hua 1
Abstract:The mature pollens of 10 maize varieties (lines) were treated with EMS. The induction effects on the germinating rates, the physiological damage of M1 and M2 were studied. The result were as follows:Pollen germinating rates decreased with the increase of EMS treatment concentrations. There were significant positive correlation between seed set percentage and the pollen germinating rates. The characterizations of M1 physiological damage were low seedling rate, long seedling period and low adult rate. The color mutation was the main type in kernel mutation of M2. In M2 seedling, leaf color mutants constituted the major part. In M2 adult, the mutation character including leaf color, plant height was observed. The germinating rates, the physiological damage of M1 and the induction effects of M2 varied with varieties at same EMS treatment concentration.
Keywords:maize  pollen  ethyl methane sulfonate  induction effects
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