牛支原体FJ-HJ分离株的分离及鉴定

从福建省患呼吸道疾病牛肺组织中分离到1株支原体,采用形态学观察、生化试验、特异性PCR检测和16S r RNA序列分析等进行鉴定,旨在明确该病病原。结果显示分离株菌落呈典型的油煎蛋状,中心有棕褐色突起,命名为FJ-HJ分离株。分离株不能水解精氨酸,不能发酵葡萄糖,不分解尿素,血细胞吸附试验和溶血试验呈阴性,胆固醇需要试验、美兰还原反应和氯化四氮唑还原反应均呈阳性;PCR反应扩增出牛支原体特异大小为1911 bp的目的片段;分离株16S r RNA基因序列与牛支原体标准株PG45的序列相似性为99.7%。研究结果表明:本次分离到的支原体为牛支原体,证实福建省存在牛支原体病的流行,为福建省牛支原体病的防治提供了科学依据。

Isolation and Identification of Mycoplasma bovis FJ-HJ Strain

One strain of Mycoplasma bovis was isolated from lung tissue of an infected beef cattle characterized by gasp and runny noses in Fujian Province. To identify this strain, colonial morphological observation, biochemical test, specific polymerase chain reaction (PCR), cloning and sequencing of 16S rRNA gene were conducted. The results showed that colonies of this strain, which was named as FJ-HJ, were like fried eggs with brown protrusion in the center. FJ- HJ could not ferment glucose, not hydrolyze arginine, and not decompose urea. Hemadsorption test and hemolysis test were negative, while cholesterol test, Meilan reduction reaction and tetrazolium nitrogen chloride reduction reaction were all positive. PCR products ofMycoplasma bovis showed the specific band of 1911 bp. Sequence analysis indicated that 16S rRNA of FJ-HJ showed 99.7% similarity with that ofMycoplasma bovis strain PG45. The results showed that the isolated strain was Mycoplasma bovis , proving the population ofMycoplasma bovis in Fujian Province. This study provides a basis for the prevention and control ofMycoplasma bovis in Fujian.