Detection of koi herpesvirus DNA in tissues of infected fish |
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| Authors: | W L Gray,L Mullis,S E LaPatra,J M Groff,& A Goodwin |
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| Affiliation: | Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, USA,;Clear Springs Foods, Inc., Research Division, Buhl, ID, USA,;Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA,;Aquaculture and Fisheries Center, University of Arkansas at Pine Bluff, Pine Bluff, AR, USA |
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| Abstract: | A newly recognized herpesvirus, koi herpesvirus or KHV, causes a lethal disease in common carp, Cyprinus carpio , and its colourful strain known as koi or fancy carp. In this study, we report new outbreaks of the disease, present initial characterization of the KHV genome, and describe assays for detection of KHV DNA in infected cells and tissues of infected fish. Restriction endonuclease (RE) profiles of viral DNA derived from two epidemiologically distinct KHV isolates were identical to each other. Cloned KHV BamHI and SphI DNA probes specifically hybridized to KHV DNA, but not to DNAs derived from a variety of other fish herpesviruses. The KHV DNA probes detected KHV DNA in tissues of experimentally infected koi fish by DNA hybridization. The KHV specific polymerase chain assays (PCR) were developed for rapid detection and confirmation of KHV DNA in tissues of infected fish. |
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| Keywords: | koi herpesvirus detection DNA probes genome analysis polymerase chain reaction (PCR) restriction endonuclease (RE) profiles |
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