猪脂肪细胞特异膜蛋白的分离鉴定

用蔗糖密度梯度离心制备脂肪细胞及其他组织细胞膜蛋白 ,通过 SDS- PAGE分离并用薄层扫描测定不同组织细胞膜蛋白的组成、相对分子质量及含量。结果表明 ,猪皮下脂肪细胞有 19种膜蛋白 ,心脏、肝脏、脾脏、肺脏、肾脏、肌肉细胞及其他脂肪细胞有 13～ 2 4种膜蛋白 ,红细胞膜有 6种膜蛋白。经 Western- blot鉴定 ,猪皮下脂肪细胞有 8种特异性膜蛋白 ,分别为 110 770、10 3910、39970、3110 0、2 94 2 0、2 5 4 80、2 35 4 0和 2 2 0 0 0。结果显示 ,脂肪细胞与其他组织细胞膜蛋白组成及含量存在较大差异

Isolation and Identification of Specific Porcine Adipocyte Membrane Protein

Porcine adipocyte membrane protein and tissue cells membrane protein were prepared using sucrose gradient centrifugation, and their conformation, molecular weight and relative abundance were analyzed by using SDS-PAGE and scanning densitometer. The results showed that 19 kinds of membrane protein were found in porcine subcutaneous adipocytes, 13-24 kinds of membrane protein were found in heart, liver, spleen, lung, kidney, skeletal muscles and other fat cells, 6 kinds of membrane protein were found in red cells. Porcine subcutaneous adipocytes contain 8 kinds of adipocyte specific membrane protein, which were identified by using Western-blot, their molecular weight being 110 770,103 910,39 970,31 100,29 420,(25 480,)23 540 and 22 000 respectively. The conformation, molecular weight and relative abundance would be different in porcine adipocytes and tissue cells membrane protein.