布鲁氏菌抗体的无标记电流型免疫传感器检测

为提高布鲁氏菌抗体检测的检测限及灵敏度,并适应定量、快速的测定要求,以便早期发现病畜,减少经济损失,采用丝网印刷金电极表面修饰巯基乙胺绑定抗原的方法,研制了一种一次性免疫传感器。通过循环伏安法表征免疫电极的反应过程,发现布鲁氏菌抗体在10-5~10-3IU/mL浓度范围内,氧化峰电流的变化值(ΔIpa)与抗体的浓度呈良好的线性关系,其相关系数r=0.999 9,检测下限为2.8×10-5IU/mL。将测定的循环伏安曲线进行半微分变换,建立半微分变化值(Δepa)与浓度的关系,在10-5~10-3IU/mL浓度范围内,半微分氧化峰电流的变化值与浓度呈线性关系,相关系数r=0.992 9,检测下限为2.7×10-6IU/mL,且在10-2~1 IU/mL浓度范围内,半微分氧化峰电流的变化值与浓度的相关系数r=0.999 2。表明半微分变换可以明显改善其检测范围和检测下限。

Label-free Amperometic Immunosensor for Detection of Brucella Antibody

In order to improve the detection sensitivity of brucella antibody and discover diseased livestock earlier for reducing economic losses, a disposable label-free immunosensor for rapid and quantitative detection was prepared. It was made by modification of cysteamine onto screen printed gold electrode surface and immobilization of antigens. By the method of cyclic voltammetry, it was shown that there is a linear relationship between the changes of peak current and the different brucella antibody concentrations within the range of 10-5~10-3IU/mL, the relevant correlation coefficient is 0.9999 and the lower detection limit is 2.8×10-5IU/mL. Furthermore, semi-differential transformation was applied to all of the cyclic voltammograms, the correlation between the changes of semi-differential values and the corresponding concentration of brucella antibody has been established, the correlation coefficient is 0.9929 and the lower detection limit is 2.7×10-6IU/mL in the range of 10-5~10-3IU/mL, and the correlation coefficient is 0.9992 in the range of 10-2~1IU/mL. It was shown that the detection range and the lower detection limit were improved greatly by the semi-differential transformation.