| 山药蛋白酶解液的分离纯化 |
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| 引用本文： | 徐梦辰,丁轲,吕莹,韩涛.山药蛋白酶解液的分离纯化[J].北京农学院学报,2014,29(3):94-96. |
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| 作者姓名： | 徐梦辰 丁轲 吕莹 韩涛 |
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| 作者单位： | 农产品有害微生物及农残安全检测与控制北京市重点实验室/北京农学院食品科学与工程学院,北京,102206;农产品有害微生物及农残安全检测与控制北京市重点实验室/北京农学院食品科学与工程学院,北京,102206;农产品有害微生物及农残安全检测与控制北京市重点实验室/北京农学院食品科学与工程学院,北京,102206;农产品有害微生物及农残安全检测与控制北京市重点实验室/北京农学院食品科学与工程学院,北京,102206 |
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| 基金项目： | 功能（保健）食品产品开发及其生物活性研究（PXM2013-014207-000048）,农产品加工及贮藏工程一北京市重点建设学科资助（PXM2013-014207-000057） |
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| 摘 要： | 以山药(Dioscorea opposite Thumb.)为原料,选取碱性蛋白酶酶解山药蛋白粗提物制备山药蛋白酶解液。分别采用纤维素DE-52阴离子交换层析和Sephadex G-50凝胶层析,对酶解制备的山药蛋白酶解液进行分离纯化。结果表明:经过纤维素DE-52阴离子交换层析,得到4个吸收峰,收集每个峰组分,其单位质量的还原能力分别为峰2(P2)>峰1(P1)>峰3(P3)>峰4(P4)。采用Sephadex G-50凝胶分别用蒸馏水和Tris-HCl缓冲液对P1和P2组分继续分离纯化,均能得到2个吸收峰(组分)。
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| 关 键 词： | 山药蛋白 酶解 纯化 |
Isolation and purification of enzymatic hydrolysis from chinese yam protein |
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| XU Mengchen,DING Ke,LV Ying,HAN Tao.Isolation and purification of enzymatic hydrolysis from chinese yam protein[J].Journal of Beijing Agricultural College,2014,29(3):94-96. |
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| Authors: | XU Mengchen DING Ke LV Ying HAN Tao |
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| Institution: | ( Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Faculty of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206,China) |
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| Abstract: | The Chinese yam protein was hydrolyzed by Alkaline protease to prepare hydrolytic products. They were isolated and purified by cellulose DE-52 anion-exchange chromatography and Sephadex G-50 gel chromatography. Four peaks of absorption were obtained with cellulose DE-52 anion-exchange chromatography, which showed the levels of reducing power as Peak 2 （P2）〉Peak 1 （P1）〉Peak 3 （P3）〉Peak 4 （P4）. Distilled water and Tris-HC1 buffer were employed for further isolation of the peaks P1 and P2 with Sephadex G-50 gel chromatography, 2 peaks of absorption were obtained, respectively. |
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| Keywords: | yam protein enzymatic hydrolysis purification |
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