| 基于RP-HPLC法的藏药麻花艽不同部位异荭草素含量的测定 |
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| 引用本文： | 聂颖杰,林鹏程.基于RP-HPLC法的藏药麻花艽不同部位异荭草素含量的测定[J].安徽农业科学,2010,38(14):7341-7342. |
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| 作者姓名： | 聂颖杰 林鹏程 |
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| 作者单位： | 青海民族大学化学与生命科学学院,青海西宁,810000 |
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| 摘 要： | 目的]建立麻花艽（Gentiana straminea Maxim.）不同部位中异荭草素的RP-HPLC检测方法。方法]色谱条件为：HypersilODS柱（4.6mm×250.0mm,5μm）,以40%甲醇-水溶液（含0.02%磷酸）等度洗脱,流速1ml/min,柱温35℃,检测波长254nm。结果]在设定的色谱条件下,异荭草素与其他组分达到基线分离,线性范围为0.0899～0.539.0μg,r=0.9999。加标回收率为99.7%,RSD为0.49%。结论]方法快速、准确,结果可靠。
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| 关 键 词： | 麻花艽 地上部位 地下部位 RP-HPLC |
Content Determination of Isoorientin in Different Parts of Tibetan Medicine Gentiana straminea Maxim. Based on RP-HPLC Method |
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| NIE Ying-jie et al.Content Determination of Isoorientin in Different Parts of Tibetan Medicine Gentiana straminea Maxim. Based on RP-HPLC Method[J].Journal of Anhui Agricultural Sciences,2010,38(14):7341-7342. |
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| Authors: | NIE Ying-jie |
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| Institution: | NIE Ying-jie et al (College of Chemistry and Life Sciences,Qinghai Nationalities University,Xining,Qinghai 810000) |
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| Abstract: | Objective]The research aimed to establish the detection method of isoorientin from different parts of Gentiana straminea Maxim. by reversed phase -HPLC. Method]The chromatographic conditions were as follows:Hypersil ODS column (4. 6 mm × 250. 0 mm,5 μm),40% methanol-H2O(containing 0. 02 % phosphoric acid)as a mobile phase,with flow rate of 1 ml/min,column temperature at 35 ℃,detection wavelength at 254 nm. Result]Under the chromatographic conditions,isoorientin and other components reached the baseline separation. The linear range was 0. 089 9 -0. 539 0 μg,r = 0. 999 9. The addition recovery was 99. 7% ,with RSD of 0. 49%. Conclusion]The method is rapid and accurate and the results are reliable. |
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| Keywords: | RP-HPLC |
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