鸡新城疫病毒融合蛋白基因的克隆及进化分析

根据GeneBank中报道的NDV融合蛋白基因(F)序列,设计了一对特异性引物,用该引物对NDVLN-SN株进行了RT-PCR扩增;将扩增得到的PCR片段纯化后与pGEM-T连接得到重组质粒pGEM-F,用于核苷酸序列测定。结果该基因ORF长1662bp,编码553个氨基酸;将LN-SN毒株与GeneBank中已报道的NDV毒株进行比较,F基因核苷酸序列的同源性在83.4%~99.9%之间,推导的F蛋白氨基酸序列的同源性在88.4%~99.8%之间。

Cloning and Sequence Analysis of Fusion Protein Gene of Newcastle Disease Virus

According to fusion protein gene sequence of Newcastle Disease virus strain reported by GeneBank database,a pair of specific primers was designed and used to amplify F gene of LN-SN strain.The positive PCR product was purified and ligatured with pGEM-T.The correct positive recombinant was used for sequencing.The open reading frame length of F gene of LN-SN strain was 1662bp and encoded 553 amino acids.The homology of nucleic acid and amino acid among the strains of NDV accessed by GeneBank database ranged from 83.4% to 99.9% and from 88.4% to 99.8%.