| 鹿骨胶原蛋白的酶解及其水解物羟自由基清除活性的研究 |
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| 引用本文： | 赵玉红,韩琳琳,高天.鹿骨胶原蛋白的酶解及其水解物羟自由基清除活性的研究[J].东北农业大学学报,2007,38(6):780-783. |
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| 作者姓名： | 赵玉红 韩琳琳 高天 |
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| 作者单位： | 东北林业大学林学院,哈尔滨,150040 |
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| 基金项目： | 黑龙江省教育厅科学技术研究指导项目 |
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| 摘 要： | 以鹿骨胶为原料,利用Alcalase碱性蛋白酶进行水解,并对水解物的.OH自由基清除活性进行了研究。结果表明,.OH清除率最佳的条件为温度65℃,pH为9,酶用量3 500 U.g-1,时间135 min,此时.OH自由基的清除率为70.8%,水解度为12.2%。。
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| 关 键 词： | 鹿骨胶 水解 清除自由基 |
| 文章编号： | 1005-9369(2007)06-0780-04 |
| 修稿时间： | 2006年9月29日 |
Study on enzymatic hydrolysis of deer bone gelatin and scavenging activity of hydrolysates on ·OH free radicals |
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| ZHAO Yuhong,HAN Linlin,GAO Tian.Study on enzymatic hydrolysis of deer bone gelatin and scavenging activity of hydrolysates on ·OH free radicals[J].Journal of Northeast Agricultural University,2007,38(6):780-783. |
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| Authors: | ZHAO Yuhong HAN Linlin GAO Tian |
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| Abstract: | The deer bone gelatin was hydrolyzed by Alcalase,and·OH free radical scavenging activity of hydrolysates was studied.The optimum hydrolysis conditions were determined: the temperature was 65 ℃,pH was 9,the amount of Alcalase was 3 500 U·g-1,and the hydrolysis time was 135 min.Under this condition,·OH free radical scavenging rate was 70.8% and the degree of hydrolysis was 12.2%. |
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| Keywords: | deer bone gelatin hydrolysis scavenging of free radical |
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