| 22份中国小麦生产主栽品种抗条锈病基因解析 |
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| 引用本文： | 徐晓丹,王明玉,冯晶,蔺瑞明,王凤涛,徐世昌.22份中国小麦生产主栽品种抗条锈病基因解析[J].植物保护学报,2018,45(1):37-45. |
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| 作者姓名： | 徐晓丹 王明玉 冯晶 蔺瑞明 王凤涛 徐世昌 |
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| 作者单位： | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193;哈尔滨医科大学(大庆)基础医学院, 黑龙江 大庆 163319,中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193,中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193,中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193,中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193,中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 |
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| 基金项目： | 国家“973”计划（2013CB127700），国家自然科学基金（31272033），国家重点研发计划（2016YFD0300705） |
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| 摘 要： | 为挖掘新的小麦抗条锈病基因,掌握小麦生产主栽品种的抗条锈病基因携带情况,有效防控小麦条锈病,采用抗性鉴定、基因推导分析和分子标记技术对22份小麦生产上主栽品种进行了研究,通过抗性鉴定比较22份小麦主栽品种与已知基因载体品种的抗谱。结果显示,共推测出14份供试品种携带已知抗条锈基因,8份供试品种携带未知抗条锈基因,是新的抗锈基因资源;聚类分析结果显示,供试22份小麦品种可分为2个大类6个亚类;利用SSR分子标记检测抗条锈病基因Yr1、Yr10和Yr24的携带情况发现,11份品种携带Yr1基因,2份品种携带Yr10基因,22份品种均不携带Yr24基因。部分生产主栽品种携带新的抗条锈病基因,表明小麦品种选育中避免了抗性基因单一化,并加强了未知基因的利用。
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| 关 键 词： | 小麦 条锈病 基因推导 聚类分析 分子检测 |
| 收稿时间： | 2017/8/4 0:00:00 |
Analysis of the stripe rust resistant genes in 22 Chinese wheat cultivars |
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| Xu Xiaodan,Wang Mingyu,Feng Jing,Lin Ruiming,Wang Fengtao and Xu Shichang.Analysis of the stripe rust resistant genes in 22 Chinese wheat cultivars[J].Acta Phytophylacica Sinica,2018,45(1):37-45. |
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| Authors: | Xu Xiaodan Wang Mingyu Feng Jing Lin Ruiming Wang Fengtao and Xu Shichang |
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| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;Department of Pathophysiology, Harbin Medical University-Daqing, Daqing 163319, Heilongjiang Province, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China and State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | In order to identify new resistance genes, understand the distribution of resistance gene to stripe rust in wheat cultivars and control wheat stripe rust, 22 wheat cultivars were studied by using resistance identification, gene postulation and molecular marker technique. Through resistance identification the resistance spectrums between the known genes-carriers and 22 wheat cultivars were tested and compared. The results showed that there were 14 cultivars with known resistance genes to stripe rust, and eight with unknown resistance genes, which were new rust resistant gene resources. The results of cluster analysis showed that 22 wheat cultivars were divided into two categories and six sub categories. Yr1, Yr10 and Yr24 genes in 22 cultivars were detected by SSR molecular markers. It was revealed that 11 cultivars carried Yr1, and two carried Yr10, while 22 did not carry Yr24. Some cultivars carried new resistance genes to stripe rust, which had important significance in avoiding the application of single resistance gene and enhancing use of unknown genes. |
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| Keywords: | wheat stripe rust gene postulation cluster analysis molecular detection |
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