新铁炮百合遗传转化体系的建立及zm401基因的导入

以培育无花粉百合新材料为目的，进行了新铁炮百合遗传转化技术的探索。结果表明，以新铁炮百合无菌苗小鳞片为外植体，在附加1.0 mg/L 6-BA 和1.0 mg/L NAA的MS基础培养基上诱导培养，通过器官直接发生途径获得再生植株，再生率达100%。农杆菌介导的遗传转化中，外植体预培养2 d，侵染5 min，共培养5 d，获得12‰的转化率。以此方法用玉米花药发育相关基因zm401转化受体材料，经PCR和PCR-Southern检测证实，zm401已经成功地整合到转化植株基因组中。该研究结果为获得无花粉百合中间材料奠定了基础。

Establishment of Regeneration System and Transformation of Zm401 Gene in Lilium longiflorum×L.formosanum

We investigated a transformation system to breed a new pollenless material of Lilium Longiflorum X L. formosanum. The results indicate that using in vitro bulb scales as explants, regeneration plants were obtained with 100% of regeneration rate through organogenesis on the basal MS media supplemented with 1.0 mg/L 6-BA and 1.0 mg/L NAA. A system for the production of transgenic plants was developed for the Lilium Longiflorum X L. formosanum by Agrobacterium-mediated transformation. The highest transformation efficiency was obtained through the following transformation procedure, after pre-culture for 3 d, the explants are immersed into bacterial suspension (OD600≈0.8) for 5 min, and then co-cultivate for 5 d. Using this system, a maize pollen specific gene, zm401, was integrated into the genome of the lily and was confirmed by PCR and PCR-Southern analysis. The results can be as a previous work to obtain indirect materials of pollenless lilies.