Abstract: | To reduce the growth period and to improve the quality of Lanzhou lily bulb, the effects of mass elements, NAA, 6-BA, medium status, sucrose and 2,4-D concentration on the formation and enlargement of small bulb of Lanzhou lily in vitro were studied. Results showed that high concentration of macro elements was beneficial to the enlargement of bulbs, but not to the induction of bulbs, the bulb diameter reached the maximum at 2MS, but the number of newly grown bulbs decreased to 4.56. When NAA concentration was 0.2 mg/L, the number of new bulbs and the bulb diameter were the highest. When 6-BA concentration was 0.1 mg/L, it was most beneficial to the formation and enlargement of bulbs, the number of new bulbs was 5.25 and the diameter of bulbs was 10.642 mg. Solid medium showed obvious effect on bulb formation, while semi-solid medium was more beneficial to bulb enlargement. When sucrose concentration was 90 g/L, the number of newly grown bulbs was the highest, the bulb diameter was the largest when sucrose concentration was 120 g/L. 0.02 mg/L 2,4-D showed better effect on bulb formation and enlargement. |