Evaluation of long-term antibody responses to two inactivated bovine viral diarrhoea virus (BVDV) vaccines |
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| Authors: | Ana M González Ignacio Arnaiz Eduardo Yus Carmen Eiras María Sanjuán Francisco J Diéguez |
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| Institution: | 1. ADSG Vacasan, Sobreira-Fornás s/n, 27500 Chantada, Spain;2. Laboratorio de Sanidad y Producción Animal de Galicia (LASAPAGA), Av. Madrid 77, 27002 Lugo, Spain;3. Unidad de Epidemiología y Sanidad Animal del Instituto de Investigación y Análisis Alimentarios, Facultad de Veterinaria de Lugo, Universidad de Santiago de Compostela, Campus Universitario s/n, 27002 Lugo, Spain;4. Departamento de Anatomía y Producción Animal, Facultad de Veterinaria de Lugo, Universidad de Santiago de Compostela, Campus Universitario s/n, 27002 Lugo, Spain;1. Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany;2. Clinic for Ruminants, LMU München, Sonnenstr. 16, D-85764 Oberschleissheim, Germany;3. Landwirtschaftskammer Nordrhein-Westfalen, Referat 34 Tiergesundheit, Siebengebirgsstrasse 200, D-53229 Bonn, Germany;1. Institute of Special Economic Animal and Plant Science, CAAS, No. 4899, Juye Street, Changchun 130122, China;2. Sinovet (Beijing) Biotechnology Co., Ltd., No. 5 Kaituo Street, Haidian District, Beijing 100085, China;3. VMRD, APAC, Zoetis, Unit 1400, 14th Floor, Sunflower Tower, No. 37 Maizidian Street, Chaoyang District, Beijing 100125, China;1. Virology Institute, CICVyA, Buenos Aires, Argentina;2. Genetic Institute “E.A. Favret”, CICVyA, Buenos Aires, Argentina;3. Faculty of Biochemistry, UBA, Buenos Aires, Argentina;4. EEA Balcarce INTA, Argentina;5. Biotechnology department, INIA, Spain |
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| Abstract: | The aim of the present study was to determine the serological response of heifers after vaccination with two inactivated bovine viral diarrhoea virus (BVDV) vaccines by means of various ELISA tests. Three dairy farms were selected from the Galicia region of Spain. In each herd, a batch of heifers to be vaccinated for the first time was selected and followed for 15 months. Heifers from farm 1 (n = 25) were vaccinated with Vaccine A, whereas heifers from farm 2 (n = 16) were vaccinated with Vaccine B. Heifers from farm 3 (n = 17), where no BVDV vaccines were used, acted as controls. Blood samples were analyzed periodically for BVDV antibodies, using five commercial ELISAs, based on BVDV p80 antigen or whole virus.At the end of the study, none of the animals vaccinated with Vaccine A seroconverted according to p80 antibody status, whereas up to 80% tested positive by ELISA against whole virus antigen. For the animals vaccinated with Vaccine B, 2/16 animals seroconverted according to p80 antibody ELISAs, whereas all had seroconverted according to the ELISA against whole virus antigen. In most cases, based on the use of ELISAs to detect specific antibodies against the p80 protein, at 15 months post-vaccination with inactivated BVDV vaccines the responses did not seem to interfere with detection of antibody to BVDV infection. However, the finding of a small proportion of vaccinated animals seropositive against BVDV p80 antigen suggests that antibodies that interfere with diagnosis of BVDV infection within the herd could exist, even when using p80 ELISAs. |
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| Keywords: | BVDV Vaccine Serology Diagnosis ELISA |
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