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Comparison of paired serum and lithium heparin plasma samples for the measurement of serum amyloid A in horses using an automated turbidimetric immunoassay
Authors:Judith Howard  Claudia Graubner
Affiliation:1. Clinical Diagnostic Laboratory, Department of Veterinary Clinical Medicine, Vetsuisse Faculty, University of Berne, Länggassstrasse 124, CH-3012 Bern, Switzerland;2. Swiss Institute of Equine Medicine (ISME), Vetsuisse Faculty, University of Berne, Länggassstrasse 124, CH-3012 Bern, Switzerland;1. Pathobiology Group, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand;2. Veterinary Teaching Hospital, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand;3. mEpilab, Infectious Disease Research Centre, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand;4. Marine Species and Threats Division, New Zealand Department of Conservation, Wellington, New Zealand;1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China;3. Department of Bioscience, Bengbu Medical College, Bengbu 233000, PR China;1. Helsinki University, Faculty of Veterinary Medicine, Department of Basic Veterinary Medicine, P.O. Box 66, 00014 Helsinki, Finland;2. ORTON Orthopaedic Hospital of ORTON Foundation, P.O. Box 29, 00281 Helsinki, Finland;3. Helsinki University Central Hospital and University of Helsinki, Department of Medicine, Biomedicum 1, P.O. Box 700, Helsinki, Finland;4. Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawinskiego 5B, 02-106 Warsaw, Poland;5. COXA Hospital for Joint Replacement, P.O. Box 652, 33101 Tampere, Finland
Abstract:
This study aimed to evaluate whether equine serum amyloid A (SAA) concentrations could be reliably measured in plasma with a turbidimetric immunoassay previously validated for equine SAA concentrations in serum. Paired serum and lithium-heparin samples obtained from 40 horses were evaluated. No difference was found in SAA concentrations between serum and plasma using a paired t test (P = 0.48). The correlation between paired samples was 0.97 (Spearman’s rank P < 0.0001; 95% confidence interval 0.95–0.99). Passing-Bablok regression analyses revealed no differences between paired samples. Bland–Altman plots revealed a positive bias in plasma compared to serum but the difference was not considered clinically significant. The results indicate that lithium-heparin plasma samples are suitable for measurement of equine SAA using this method. Use of either serum or plasma allows for greater flexibility when it comes to sample collection although care should be taken when comparing data between measurements from different sample types.
Keywords:Acute phase proteins  Anticoagulant effects  Horse  Plasma  Serum amyloid A
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