一种利用气相色谱快速检测瘤胃体外发酵液中三甲胺浓度的方法

本试验旨在建立一种利用气相色谱快速检测瘤胃体外发酵液中三甲胺浓度的方法.瘤胃体外发酵试验设置2个组,对照组(n=4)和试验组(n=4),试验组每个发酵瓶中添加0.0414 g三甲胺盐酸盐,发酵24 h.采用10 mol/L氢氧化钾溶液对发酵液样品进行预处理,使用WEL-PEG20M色谱柱和氢火焰离子检测器对三甲胺进行分...

A Method for Rapid Detection of Concentration of Trimethylamine in Rumen in Vitro Fermentation Liquid by Gas Chromatography

This study aimed to establish a method for rapid determination of concentration of trimethylamine(TMA)in rumen in vitro fermentation liquid by gas chromatography.The rumen in vitro fermentation experiment was set two groups:control group(n=4)and experimental group(n=4).Each fermentation bottle in the experimental group was added with 0.0414 g trimethylamine hydrochloride,and all bottles were incubated 24 h.The fermentation liquid was pretreated with 10 mol/L potassium hydroxide(KOH).TMA was separated in WME-PEG20M column and detected by flame ionization detector.A series of TMA standard solution were prepared with the fermentation medium without background TMA to prepare standard curve.The results showed that the standard curve of TMA had a good linear relationship between 0.1 to 1.0 mg/mL(R 2≥0.993),the average recovery rates were 98.44%to 100.27%,and the detection limit of TMA was 0.01 mg/mL.TMA was not detectable in the fermentation liquid at 0 and 24 h in the control group.In the experimental group,the TMA concentrations were 0.65 mg/mL at 0 h and 0.12 mg/mL at 24 h,suggesting that TMA was greatly consumed by rumen microorganisms.In conclusion,the TMA detection method established in this study is simple procedure,relatively high accuracy and sensitivity,and can quickly,accurately monitor the changes of TMA concentration in the fermentation liquid.This method provides a fast and efficient detection method for the study of the TMA metabolism by rumen microorganisms.